Encapsulation of Bifidobacterium pseudocatenulatum Strain G4 within Bovine Gelatin-Genipin-Sodium Alginate Combinations: Optimisation Approach Using Face Central Composition Design-Response Surface Methodology (FCCD-RSM)
Table 3
Experimental design and results using face-centered full factorial design (FCCD): bovine gelatin, sodium alginate, and genipin.
Run
Bovine gelatin (% w/v), x1
Genipin (mM), x2
Sodium alginate (% w/v), x3
Responses1
BS2 before (g), y1
BS after SGF3 (g), y2
BS after SIF4 (g), y3
EY5 (%), y4
13
10
10
1
91.4
177.4
66.2
35.2
14
10
10
5
292.9
654.9
120.2
43.8
15
10
30
3
178.1
269.4
75.1
41.6
16
10
50
1
154.7
249.0
102.2
39.6
12
10
50
5
532.9
1025.2
228.0
44.9
3
11.5
10
3
1860.0
3797.4
411.0
54.4
1
11.5
30
1
1567.3
2727.4
309.1
50.1
2
11.5
30
3
2462.3
4604.6
380.1
62.4
6
11.5
30
3
2678.5
4551.7
372.1
65.3
11
11.5
30
3
2399.6
4469.3
298.0
61.0
7
11.5
30
3
2362.2
5288.1
382.1
62.2
19
11.5
30
3
2148.8
4463.9
375.1
60.1
18
11.5
30
5
2962.7
4928.0
417.1
65.7
10
11.5
50
3
2919.0
4199.3
420.1
61.6
8
13
10
1
336.5
396.9
199.1
55.4
17
13
10
5
4310.0
7703.5
249.0
61.5
9
13
30
3
3111.3
5415.1
206.1
58.1
5
13
50
1
349.6
542.9
260.4
52.2
4
13
50
5
4435.3
6975.5
476.1
54.3
1All factorial and axial points are means of duplicate; 2BS, bead strength; 3SGF, simulated gastric fluid; 4SIF, simulated intestinal fluid; 5EY, encapsulation yield.
