International Journal of Microbiology / 2019 / Article / Tab 4 / Research Article
Encapsulation of Bifidobacterium pseudocatenulatum Strain G4 within Bovine Gelatin-Genipin-Sodium Alginate Combinations: Optimisation Approach Using Face Central Composition Design-Response Surface Methodology (FCCD-RSM) Table 4 ANOVA and regression analysis for the responses of bead strength before (y 1 ) and after SGF (y 2 )/SIF exposure (y 3 ) as well as encapsulation yield (y 4 ).
Source Sum of squares DF1 Mean square F value valuey 1 2368.9 1129.27x 1 154.09x 2 1003.4x 3 989.7 934.9x 1 x 3 Model 3.5E + 007 5 6.938E + 006 70.7 <0.0001a Residual 1.3E + 006 13 98088.27 Lack of fit 1.1E + 006 9 1.255E + 005 3.5 0.1226 Pure error 1.5E + 005 4 36366.13 Factorb /intercept x 1 1.3E + 007 1 1.275E + 007 130.1 <0.0001 x 2 2.4E + 005 1 2.374E + 005 2.4 0.0438 x 3 1.0E + 007 1 1.007E + 007 102.6 <0.0001 4.6E + 006 1 4.639E + 006 47.3 <0.0001 x 1 x 3 6.9E + 002 1 6.993E + 002 71.2 <0.0001 R 2 = 96.45%, adequate precision = 25.99y 2 4336.6 1865.8x 1 26.8x 2 1719.5x 3 1995.7 1560.6x 1 x 3 Model 1.0E + 008 5 2.055E + 007 52.8 <0.0001a Residual 5.1E + 008 13 3.893E + 005 Lack of fit 4.6E + 006 9 5.086E + 005 4.2 0.0898 Pure error 4.8E + 005 4 1.207E + 005 Factorb /intercept x 1 3.5E + 007 1 3.5E + 007 89.4 <0.0001 x 2 6852.4 1 6852.4 1.0 0.0165 x 3 2.9E + 007 1 2.9E + 007 75.9 <0.0001 1.9E + 007 1 1.9E + 007 48.5 <0.0001 x 1 x 3 1.9E + 007 1 1.9E + 007 50.1 <0.0001 R 2 = 95.31%. Adequate precision = 20.6y 3 359.9 79.9x 44.1 55.3x 3 212.0 62.9 29.7x 2 x 3 Model 2.8E + 005 6 46605.7 30.7 <0.0001a Residual 1444.0 12 1203.5 Lack of fit 9346.4 8 1168.3 0.9 0.5770 Pure error 5095.7 4 1273.9 Factorb /intercept x 1 63854.5 1 63854.5 53.1 <0.0001 x 2 19458.7 1 19458.7 16.2 0.0017 x 3 30629.6 1 30629.6 25.2 0.0003 1.4E + 005 1 1.4E + 005 25.5 <0.0001 12503.9 1 12503.9 117.9 0.0073 x 2 x 3 7065.0 1 7065.0 5.9 0.0321 R 2 = 95.09%, adequate precision = 19.27y 4 60.3 7.7x 1 0.2x 2 3.8x 3 11.7Model 1371.9 4 343.0 28.1 <0.0001 Residual 170.8 14 12.2 Lack of fit 155.9 10 15.8 4.0 0.0959 Pure error 15.4 4 3.86 Factorb /intercept x 1 584.9 1 584.9 47.9 <0.0001 x 2 0.5 1 0.5 0.1 0.8340 x 3 141.3 1 141.3 11.6 0.0043 645.2 1 645.2 52.8 <0.0001 R 2 = 88.93%, adequate precision = 15.11
a Significant at α = 0.05. 1 DF, degree of freedom; x 1 , bovine gelatin (% w/v); x 2 , genipin (mM); x 3 , sodium alginate (% w/v).