International Journal of Microbiology

Research Article

Effectiveness of BOX-PCR in Differentiating Genetic Relatedness among Salmonella enterica Serotype 4,[5],12:i:- Isolates from Hospitalized Patients and Minced Pork Samples in Northern Thailand

Table 1

Primers used in this study.
PrimerGenesSequence (5′ ⟶ 3′)Size of PCR product (bps)Primer concentration (pmol/μl)Reference
Antibiotic-resistant determinants
Multiplex 1 blaTEM variants including blaTEM-1 and blaTEM-2, blaSHV variants including blaSHV-1, and blaOXA-1-like including blaOXA-1, blaOXA-4, and blaOXA-30
blaTEM_fblaTEMCATTTCCGTGTCGCCCTTATTC8000.4[22]
blaTEM_r CGTTCATCCATAGTTGCCTGAC 0.4[22]
blaSHV_fblaSHVAGCCGCTTGAGCAAATTAAAC7130.4[22]
blaSHV_r ATCCCGCAGATAAATCACCAC 0.4[22]
blaOXA_fblaOXAGGCACCAGATTCAACTTTCAAG5640.4[22]
blaOXA_r GACCCCAAGTTTCCTGTAAGTG 0.4[22]
Multiplex 2 blaCTX-M group 1 and group 9: variants of blaCTX-M group 1 including blaCTX-M-1, blaCTX-M-3, and blaCTX-M-15 and variants of blaCTX-M group 9 including blaCTX-M-9 and blaCTX-M-14
CTX 1_fblaCTX-M group 1TTAGGAARTGTGCCGCTGYAb6880.4[22]
CTX 1_r CGATATCGTTGGTGGTRCCATb 0.2[22]
CTX 9_fblaCTX-M group 9TCAAGCCTGCCGATCTGGT5610.4[22]
CTX 9_r TGATTCTCGCCGCTGAAG 0.4[22]
Multiplex 3 blaCTX-M group 1 and group 9
CTX 1_fblaCTX-M group 1TTAGGAARTGTGCCGCTGYAb6880.4[22]
CTX 1_r CGATATCGTTGGTGGTRCCATb 0.2[22]
CTX 9_fblaCTX-M group 9TCAAGCCTGCCGATCTGGT5610.4[22]
CTX 9_r TGATTCTCGCCGCTGAAG 0.4[22]
Multiplex 4 mcr variants including mcr 1, 3, and 4
mcr 1_fmcr-1AGTCCGTTTGTTCTTGTGGC3200.25[23]
mcr 1_r AGATCCTTGGTCTCGGCTTG 0.25[23]
mcr 3_fmcr-3AAATAAAAATTGTTCCGCTTATG9290.25[23]
mcr 3_r AATGGAGATCCCCGTTTTT 0.25[23]
mcr 4_fmcr-4TCACTTTCATCACTGCGTTG11160.25[23]
mcr 4_r TTGGTCCATGACTACCAATG 0.25[23]
Molecular typing
ERIC_fERIC-PCRATGTAAGCTCCTGGGGATTCAC 25[24]
ERIC_r AAGTAAGTGACTGGGGTGAGCG 25[24]
GTG_frGTG5-PCRGTGGTGGTGGTGGTG 25[15]
BOXA1R_frBOX-PCRCTACGGCAAGGCGACGCTGACG 20[25]
REP_fREP-PCRIIIGCGCCGICATCAGGC 25[16]
REP_r ACGTCTTATCAGGCCTAC 25[16]
bY = T or C; R = A or G; S = G or C; D = A or G or T.