Effectiveness of BOX-PCR in Differentiating Genetic Relatedness among Salmonella enterica Serotype 4,[5],12:i:- Isolates from Hospitalized Patients and Minced Pork Samples in Northern Thailand

Table 4

Differentiation into clusters by the four molecular typing methods of Salmonella isolates of the same serotype collected from the same source and two different sources.

Percent similarity^a	Serotype (number of isolates)	Source	Number of clusters^a (number of isolates in each cluster), discriminatory index^b
			BOX-PCR	GTG5-PCR	REP-PCR	ERIC-PCR
50%	Typhimurium (6)	Minced pork	1 (6), 0	3 (3, 2, 1), 0.733	1 (6), 0	1 (6), 0
	S. 4,[5],12:i:- (6)	Minced pork	3 (3, 2, 1), 0.7333	2 (4, 2), 0.5333	1 (6), 0	1 (6), 0
	S. 4,[5],12:i:- (12)	Hospitalized patients	2 (10, 2), 0.303	3 (6, 5, 1), 0.6212	2 (6, 6), 0.5455	2 (8, 4), 0.4848
80%	Typhimurium (6)	Minced pork	3 (4, 1, 1), 0.6	3 (3, 2, 1), 0.7333	2 (3, 3), 0.6	1 (6), 0
	S. 4,[5],12:i:- (6)	Minced pork	4 (2, 2, 1, 1), 0.8667	3 (2, 2, 2), 0.8	2 (4, 2), 0.5333	1 (6), 0
	S. 4,[5],12:i:- (12)	Hospitalized patients	7 (3, 2, 2, 2, 1, 1, 1), 0.9091	6 (4, 2, 2, 2, 1, 1), 0.8636	4 (5, 3, 3, 1), 0.7576	6 (6, 2, 1, 1, 1, 1), 0.7576

^aFrom phylogenetic tree (Figure 2). ^b[28].