Heterologous Ectoine Production in Escherichia coli: Optimization Using Response Surface Methodology

Figure 4

HPLC analysis of authentic ectoine and ectoines produced by the recombinant E. coli. (a) Authentic ectoine (Fluka, Sigma). (b) Intracellular ectoine. (c) Extracellular ectoine. Ectoine is confirmed by the peak at the retention time of about 1.2 minutes (circle).

(a)

(b)

(c)