Heterologous Ectoine Production in Escherichia coli: Optimization Using Response Surface Methodology

Table 2

Production of ectoine by the recombinant E. coli.

Experimental repetition	Biomass conc. (mg/mL)	Ectoine conc. (g/L)		Productivity (mg ectoine/g cdw)		Release of ectoine (%)
		Intracellular	Extracellular	Intracellular	Extracellular
1	3.5	0.074	0.22	21.4	63.6	75
2	3.3	0.070	0.23	21.0	70.3	77
3	3.4	0.070	0.25	20.7	72.9	78
Average standard deviation	3.4	0.071	0.23	21.0	68.9	77
	0.08	0.002	0.01	0.3	4.8	1.6

Release of ectoine is stated as the percentage of extracellular ectoine per total ectoine produced (extracellular + intracellular ectoines).