Mycobacterium bovis BCG Surface Antigens Expressed under the Granuloma-Like Conditions as Potential Inducers of the Protective Immunity

Figure 1

Binding and invasion assays of M. bovis BCG exposed to granuloma-like (pH 6.0, 0.3 M dextrose, anaerobic) and regular lab (pH 7.2, 20% O₂) conditions. (a) Percentage of bacterial binding to A549 lung epithelial cells. (b) Percentage of bacterial binding to THP-1 macrophages. (c) Percentage of bacterial invasion of A549 cells. (d) Percentage of bacterial invasion of THP-1 cells. Results represent mean ± standard error of three independent experiments. , and . Percentage of binding and invasion is calculated from the total M. bovis BCG inoculum added to cell monolayers at 0 time point.

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