Mycobacterium bovis BCG Surface Antigens Expressed under the Granuloma-Like Conditions as Potential Inducers of the Protective Immunity

Figure 2

M. bovis BCG surface proteins expressed in different conditions. (a) Venn diagram showing the number of overlapping and unique set of bacterial surface proteins synthesized in granuloma-like and regular lab conditions. (b) Western blotting of M. smegmatis cell lysate overexpressing Mb1524, Mb01_03198, PhoU1, and hbhA proteins using anti-His monoclonal antibody. Lane 1, pMV261- PhoU1; Lane 2, pMV261-Mb01_03198; Lane 3, pMV261-hbhA; Lane 4, pMV261-Mb1524; Lane 5, pMV261 control. Arrows indicate expressed proteins.

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