Mycobacterium bovis BCG Surface Antigens Expressed under the Granuloma-Like Conditions as Potential Inducers of the Protective Immunity

Table 1

Primers used for construction of M. smegmatis clones overexpressing M. bovis BCG surface antigens in the pMV261 vector. His-tag was incorporated in the primer.

Genes	Primers
Mb1524	5′-TTTTTGAATTCCATCATCATCATCATCATCAAGGAGCCGTTGCT-3′; 5′-TTTTTGTCGACCTATTGAGTCAACCTGGGGGG-3′
Mb01_03198	5′-TTTTTGAATTCCATCATCATCATCATCATGACACAACTGTC-3′; 5′-TTTTTGTCGACCTAAGCGTCGATCCC-3′
PhoU1	5′-TTTTTGAATTCCATCATCATCATCATCATCGGACGGTCTAT-3′; 5′-TTTTTGTCGACTCAGTAAGTGGAAATCTCGTCCT-3′
HBHA	5′-TTTTTGAATTCCATCATCATCATCATCATGCTGAAAACTCGAAC-3′; 5′-TTTTTGTCGACCTACTTCTGGGTGACCTTCTT-3′