Functional Gene Diversity of Selected Indigenous Hydrocarbon-Degrading Bacteria in Aged Crude Oil
Table 3
Baseline properties/characteristics of the study site.
Parameters
Method
DPR intervention limit
Concentration/quantity
pH
APHA 4500H+B
NA
5.97
E. conductivity (μs/cm)
Cond. Meter
NA
60
Moisture content (%)
BS 1377-2:1990 clause 1
NA
2.40
Nitrate (NO3) (mg/kg)
APHA 4500- NO3−
NA
0.46
Phosphate (P2O5) (mg/kg)
APHA 4500-P
NA
18.24
Total organic carbon (%)
BS 1377-2:1990 clause 3
NA
31.4
Nickel (mg/kg)
ASTM D 1886
10
2.420
Lead (mg/kg)
ASTM D 3559
380
1.283
Zinc (mg/kg)
ASTM D 1691
210
52.50
TPH (mg/kg)
EPA 8015
5000
8695.77231
PAH (mg/kg)
EPA 8260
40
989.11889
THB
Spread plate
NA
3.11 × 108
HUB
Spread plate
NA
2.43 × 108
Method source: the American Public Health Association (APHA) 20th Edition 2008, American Society for for Testing and Materials (ASTM), EPA: Environmental Protection Agency. Keys: Cond. Meter: conductivity meter, NA: not available, BS: British Standard, Cfu/g: colony forming unit per gram.