Research Article

Functional Gene Diversity of Selected Indigenous Hydrocarbon-Degrading Bacteria in Aged Crude Oil

Table 3

Baseline properties/characteristics of the study site.

ParametersMethodDPR intervention limitConcentration/quantity

pHAPHA 4500H+BNA5.97
E. conductivity (μs/cm)Cond. MeterNA60
Moisture content (%)BS 1377-2:1990 clause 1NA2.40
Nitrate (NO3) (mg/kg)APHA 4500- NO3−NA0.46
Phosphate (P2O5) (mg/kg)APHA 4500-PNA18.24
Total organic carbon (%)BS 1377-2:1990 clause 3NA31.4
Nickel (mg/kg)ASTM D 1886102.420
Lead (mg/kg)ASTM D 35593801.283
Zinc (mg/kg)ASTM D 169121052.50
TPH (mg/kg)EPA 801550008695.77231
PAH (mg/kg)EPA 826040989.11889
THBSpread plateNA3.11 × 108
HUBSpread plateNA2.43 × 108

Method source: the American Public Health Association (APHA) 20th Edition 2008, American Society for for Testing and Materials (ASTM), EPA: Environmental Protection Agency. Keys: Cond. Meter: conductivity meter, NA: not available, BS: British Standard, Cfu/g: colony forming unit per gram.