Supershed Escherichia coli O157:H7 Has Potential for Increased Persistence on the Rectoanal Junction Squamous Epithelial Cells and Antibiotic Resistance

Table 1

MIC profiles of all isolates with an intermediate to resistant phenotype in the AST assay.

Strains^c	Description	MIC profile^a (μg/mL) (using Etest® MIC strips^b)
		XL	AM	AZ	CL	CT	FX	GM	NA	SM	SX	TE
E. coli ATCC25922	Test control	8	4	7	8	0.09	3	0.38	3	2	64	2
Sakai	Control O157	6	3	4	8	0.09	6	0.25	4	3	128	3
EDL933	Control O157	4	3	4	8	0.08	8	0.25	4	2	64	3
EC4115	Control O157	S	S	1	6	S	3	S	S	S	S	3
JEONG-1266	Control SS-O157	6	3	4	8	0.06	6	0.38	4	6	128	2
SS-17	Control SS-O157	4	2	3	6	0.06	4	0.38	3	6	96	3
SS-52	Control SS-O157	6	3	3	8	0.08	6	0.38	6	6	96	3
870-1	E. coli	S	S	1	S	S	S	S	S	1	S	6
870-2	E. coli	8	4	0.75	256	S	S	S	S	384	1024	128
870-4	E. coli	3	2	4	4	0.04	2	0.38	4	1.5	48	6
888-1	E. coli	S	S	1.5	S	S	S	S	S	S	S	16
888-2	E. coli	6	3	3	8	0.09	6	0.25	6	6	1024	4
888-4	E. coli	S	S	1.5	S	S	S	S	S	1	128	S
912-1	E. coli	S	S	1.5	S	S	S	S	6	2	1024	S
912-2	E. coli	S	S	1.5	S	S	S	S	S	3	192	96
912-4	E. coli	3	2	8	8	0.03	3	0.38	4	8	1024	48
914-1	E. coli	S	S	2	S	S	4	S	S	1	S	4
914-2	E. coli	S	6	1	S	S	S	S	S	1	S	S
914-3	E. coli	8	8	2	S	S	S	S	S	1.5	128	S
914-4	E. coli	8	6	2	S	S	S	S	S	1.5	S	S
887-1	E. coli (STEC)	4	4	7	6	0.06	3	0.38	4	6	48	3
887-2	E. coli (STEC)	6	4	4	6	0.09	3	0.38	4	6	48	3
887-3	E. coli (STEC)	8	4	4	6	0.08	2	0.38	4	6	48	3
887-4	E. coli (STEC)	6	4	4	6	0.06	3	0.38	6	2	48	3
C-1	SS-O157	6	4	3	6	0.09	6	0.25	4	4	128	2
C-2	SS-O157	6	3	4	8	0.06	6	0.38	4	6	64	2
C-4	SS-O157	S	S	1	S	S	S	S	S	S	192	3
C-5	SS-O157	S	S	1	6	S	S	S	S	S	192	S
C-7	SS-O157	6	3	3	8	0.06	6	0.38	8	4	128	3
C-9	SS-O157	S	S	1	S	0.06	S	S	S	1.5	S	S
C-11	SS-O157	S	S	1	6	0.05	S	S	S	S	64	S
C-12	SS-O157	S	S	1	8	0.05	S	S	S	S	128	3
C-13	SS-O157	S	S	1	S	S	S	S	S	S	192	S
C-14	SS-O157	S	S	1	6	S	S	S	S	3	128	S
C-18	SS-O157	S	S	1.5	S	S	S	S	S	3	96	S
C-19	SS-O157	S	S	1.5	4	0.05	S	S	S	4	S	S
C-22	SS-O157	S	S	0.75	S	S	S	0.25	S	2	96	S
C-32	SS-O157	S	S	1	8	S	S	S	S	2	S	S
C-38	SS-O157	S	S	1	8	0.06	S	S	S	2	192	4
C-40	SS-O157	4	4	4	8	0.06	6	0.38	4	6	64	2
C-45	SS-O157	6	4	3	6	0.11	6	0.25	4	6	192	3
C-49	SS-O157	6	4	4	8	0.06	4	0.38	4	6	128	3
C-66	SS-O157	S	S	1	8	0.06	S	S	6	S	1024	S
C-77	SS-O157	6	3	3	6	0.06	4	0.5	4	8	48	3
C-80	SS-O157	6	3	3	8	0.06	6	0.25	8	0.75	64	3
C-85	SS-O157	S	S	1	S	0.05	S	S	S	S	256	S
C-87	SS-O157	S	S	1	S	0.05	S	S	S	1	1024	S
C-88	SS-O157	S	4	1	S	0.06	S	S	S	1	256	S
C-90	SS-O157	S	S	1	S	S	S	S	S	S	256	S
C-99	SS-O157	4	3	4	6	0.08	6	0.25	6	1.5	64	3
C-104	SS-O157	4	3	4	8	0.09	6	0.25	6	24	1024	256

^aMIC Profile: MIC of 11 antibiotics was determined in all the isolates that were either Intermediate or Resistant on AST assay; remaining isolates are labelled S against the MIC strip to which the isolates were susceptible in AST assay. ^bAntibiotic MIC Strips: XL = Amoxicillin/Clavulanic acid; AM = Ampicillin; AZ = Azithromycin; CL = Chloramphenicol; CT = Cefotaxime (in place of Ceftiofur); FX = Cefoxitin; GM = Gentamicin; NA = Nalidixic Acid; SM = Streptomycin; SX = Sulfisoxazole; TE = Tetracycline. ^cOnly one representative isolate was used for MIC testing if AST profile was the same.