In Vitro Antimicrobial Activity of Piper retrofractum Fruit Extracts against Microbial Pathogens Causing Infections in Human and Animals

Table 3

Minimum inhibitory concentration (MIC) of crude extract by microdilution assay.

Antimicroorganism	Weight of crude extract from organic solvent (mg/ml)
	Hex	Iso	Dic	Ace	Met
E. coli	Ni	18.00	80.00	36.00	22.50
B. subtilis	Ni	9.00	80.00	71.50	5.50
E. faecalis	ni	71.50	40.00	4.50	45.00
K. pneumoniae	ni	Ni	ni	143.50	90.00
P. aeruginosa	ni	Ni	ni	143.50	179.50
S. typhi	ni	9.00	10.00	9.00	11.00
S. aureus	ni	35.50	80.00	71.50	45.00
V. parahaemolyticus XN89	ni	71.50	80.00	71.50	45.00
V. paraparahaemolyticus 5HP	ni	18.00	40.00	71.50	22.50
C. albicans	2.50	10.00	15.00	1.00	0.50

Hex = hexane, Iso = isopropanol, Dic = dichloromethane, Ace = acetonitrile, Met = methanol, and ni = no inhibition.