Research Article

The Improvement of Bioethanol Production by Pentose-Fermenting Yeasts Isolated from Herbal Preparations, the Gut of Dung Beetles, and Marula Wine

Table 6

Fermentation parameters of the adapted and parental yeast strains from M. caribbica Mu 2.2f with D-xylose, L-arabinose, or a mixture of the pentose sugars at 35°C with or without acetic acid.

Fermentation parametersAbsence of acetic acid3 g/L acetic acid
AdaptedParentalAdapted
D-xyloseL-arabinoseMixtureD-xyloseL-arabinoseMixtureD-xyloseL-arabinose

Maximum ethanol (g/L)1.7 ± 0.5a3.0 ± 0.4c1.9 ± 0.2b1.5 ± 0.7a1.0 ± 0.11a1.8 ± 0.5b0.8 ± 0.1b3.6 ± 0.1b
Ethanol yield (g/g)0.0420.1480.0590.0440.0760.0520.0400.181
Maximum arabitol (g/L)16.8 ± 1.4d4.7 ± 3.1d7.4 ± 0.14c2.2 ± 0.1b20.2 ± 1.5e
Xylitol (g/L)2.8 ± 0.4b4.6 ± 1.6e8.5 ± 0.4b6.5 ± 1.3d3.8 ± 0.7b
Arabitol yield (g/g)0.4940.3470.3250.3810.657
Xylitol yield (g/g)0.060.0840.200.1500.172
Ethanol productivity (g/L/h)0.0710.0620.0530.0630.0430.0400.0200.100

Ethanol and L-arabitol yields are reported at maximum ethanol or L-arabitol concentrations. a: after 24 hours of fermentation; b: after 36 hours of fermentation; c: after 48 hours of fermentation; d: after 72 hours of fermentation; e: after 96 hours of fermentation.