Antifungal Activity of Thai Cajuput Oil and Its Effect on Efflux-Pump Gene Expression in Fluconazole-Resistant Candida albicans Clinical Isolates

Table 1

Primers for the CDR1, CDR2, MDR1, and ACT1 genes for PCR and qRT-PCR [10].

Genes	Primers	Sequences	Primer concentration (μM)	Annealing temperature (°C)	Amplicon size (bp)	GenBank   accession no.
CDR1	Forward	5’-TGCCAAACAATCCAACAA-3’	0.5	58	112	X77589
	Reverse	5’-CGACGGATCACCTTTCATACGA-3’	0.5
CDR2	Forward	5’-AAGGTTTTGATGCTACTGC-3’	0.5	55	125	U63812
	Reverse	5’-GTCGGACATGTGGCTCAAA-3’	0.5
MDR1	Forward	5’-GTGTTGGCCCATTGGTTTTCAGTC-3’	0.7	58	114	X53823
	Reverse	5’-CCAAAGCAGTGGGGATTTGTAG-3’	0.7
ACT1	Forward	5’-AAGAATTGATTTGGCTGGTAGAGA-3’	0.5	58	179	X16377
	Reverse	5’-TGGCAGAAGATTGAGAAGAAGTTT-3’	0.5