Research Article

Transcriptomic Response in Pseudomonas aeruginosa towards Treatment with a Kaempferol Isolated from Melastoma malabathricum Linn Leaves

Table 5

List of the group V genes with downregulated expression at 24 h.

Genes24 h (fold change) valueDescriptionFunctional class

pchR−3.1162.23E − 15Transcriptional regulator PchRTRs
PA0471−2.8631.61E − 05Fe2+-dicitrate sensor, membrane componentTCRSs; MPs; TRs
fiuI−2.1711.62E − 03Fe2+-dicitrate sensor, membrane componentTRs
PA1300−2.2015.72E − 09Sigma-70 factor, ECF subfamilyTRs
PA13012.3860.014Probable transmembrane sensorMPs; TRs
PA3899−3.4810Probable sigma-70 factor, ECF subfamilyTRs
PA3900−2.5050.019Fe2+-dicitrate sensor, membrane componentMPs; TRs
PA4895−5.9651.26E − 09Fe2+-dicitrate sensor, membrane componentMPs; TRs
PA4896−3.6448.33E − 11Sigma-70 factor, ECF subfamilyTRs
PA0149−3.8591.15E − 08Probable sigma-70 factor, ECF subfamilyTRs
femI−3.6280ECF sigma factor, FemITRs
PA2896−2.4950Probable sigma-70 factor, ECF subfamilyTRs
tonB1−2.0670Periplasmic protein TonB, links inner and outer membranesTSMs
PA4156−11.360Probable TonB-dependent receptorTSMs
fumC1−4.5990Fumarate hydrataseEM
sodM−3.9550Superoxide dismutaseAP
hemO−3.5810Heme oxygenaseBCPGCs
PA0853−3.2422.66E − 13OxidoreductasePEs
PA3768−2.6220Probable metallo-oxidoreductasePEs
phzA1−4.2951.53E − 07Probable phenazine biosynthesis proteinSFs
phzB1−4.708−4.708Probable phenazine biosynthesis proteinSFs
phzC1−2.577−2.577Phenazine biosynthesis protein PhzCSFs
phzA2−2.625−2.625Probable phenazine biosynthesis proteinSFs
phzB2−3.848−3.848Probable phenazine biosynthesis proteinSFs
phzM−2.1251.14E − 10Probable phenazine-specific methyltransferasePEs
phzS−2.2410Flavin-containing monooxygenasePEs
secA−2.1660Secretion protein SecAPSEA
secB−2.5790Secretion protein SecBPSEA
secD−3.2340Secretion protein SecDPSEA; MPs
mexG−2.1197.29E − 03Membrane proteinMPs
mexH−9.0880Probable resistance-nodulation-cell division (RND) efflux membrane fusion protein precursorTSMs
mexI−5.7580Probable resistance-nodulation-cell division (RND) efflux transporterTSMs; MPs
opmD−3.2410Outer membrane protein precursorTSMs; MPs
lpxB−2.1487.24E − 03Lipid A-disaccharide synthaseCWLC
lpxA−2.2740UDP-N-acetylglucosamine acyltransferaseCWLC
waaP−3.1742.75E − 14Lipopolysaccharide kinase WaaPCWLC
waaG−2.4370UDP-glucose:(heptosyl) LPS alpha 1,3-glucosyltransferase WaaGCWLC
waaF−2.2831.48E − 08Heptosyltransferase IICWLC
PA4998−2.4826.80E − 12Aminoglycoside 3′-phosphotransferase (APH) and choline kinase familyCWLC
PA5007−3.1244.80E − 08Mn2+−dependent serine/threonine protein kinasePEs
PA5008−3.1877.22E − 15RIO-like serine/threonine protein kinase fused to N-terminal HTH domainPEs
rmlA−2.5540Glucose-1-phosphate thymidylyltransferaseCWLC
pilD−2.3770Type 4 prepilin peptidase PilDSFs; PSEA; MA
pilF−2.0530Type 4 fimbrial biogenesis protein PilFPSEA; MA
pilM−3.0740Type 4 fimbrial biogenesis protein PilMMA
pilN−3.8710Type 4 fimbrial biogenesis protein PilNMA
pilO−4.4760Type 4 fimbrial biogenesis protein PilOMA
pilP−3.9560Type 4 fimbrial biogenesis protein PilPMA
pilQ−3.2190Type 4 fimbrial biogenesis outer membrane protein PilQ precursorMA
pilU−2.2260Twitching motility protein PilUMA
pilV−2.3820Type 4 fimbrial biogenesis protein PilVMA
pilW−2.5160Type 4 fimbrial biogenesis protein PilWMA
pilX−2.3660Type 4 fimbrial biogenesis protein PilXMA
pilY1−2.0250Type 4 fimbrial biogenesis protein PilY1MA
pilG−2.7130Twitching motility protein PilGTCRSs; MA; CT
pilH−3.1120Twitching motility protein PilHTCRSs; MA; CT
pilI−2.7312.20E − 09Twitching motility protein PilIMA; CT
pilJ−5.2820Twitching motility protein PilJMA; CT
Vfr−2.0470Transcriptional regulator vfrTRs
chpA−2.1240Component of chemotactic signal transduction systemTCRSs; MA; CT
chpB−2.3151.15E − 05Probable methylesteraseCT
fliC−2.1450Flagellin type BMA
rpsK−2.592030S ribosomal protein S11TPTMD
rplA−2.375050S ribosomal protein L1TPTMD
glnS−2.1020Glutaminyl-tRNA synthetaseTPTMD; AABM
glyS−2.1622.11E − 13Glycyl-tRNA synthetase beta chainTPTMD; AABM
leuS−2.1310Leucyl-tRNA synthetaseTPTMD; AABM
lysS−2.380Lysyl-tRNA synthetaseTPTMD; AABM
proS−2.7640Prolyl-tRNA synthetaseTPTMD; AABM
valS−2.130Valyl-tRNA synthetaseTPTMD; AABM
aspS−2.1290Aspartyl-tRNA synthetaseT-RNA-PD; TPTMD
hisF1−3.5991.02E − 11Imidazole glycerol-phosphate synthase, cyclase subunitAABM
hisG−2.8632.04E − 10ATP-phosphoribosyltransferaseAABM
argB−2.4360Acetylglutamate kinaseAABM
argG−2.4280Argininosuccinate synthaseAABM
argH−2.1270Argininosuccinate lyaseAABM
cysM−3.4360Cysteine synthase BAABM
trpA−4.4050Tryptophan synthase alpha chainAABM
trpB−6.5270Tryptophan synthase beta chainAABM
hslU−4.2550Heat shock protein HslUCHSPs
hslV−3.7420Heat shock protein HslVCHSPs
htpG−2.8350Heat shock protein HtpGCHSPs
htpX−2.5570Heat shock protein HtpXAP
dnaA−2.6310Chromosomal replication initiation proteinDNA-RRMR
dnaJ−2.5280DnaJ proteinDNA-RRMR; CHSPs; AP
dnaK−3.0870DnaK proteinDNA-RRMR; CHSPs; AP
holC−2.7015.25E − 09DNA polymerase III, chi subunitDNA-RRMR
mutL−2.5630DNA mismatch repair protein MutLDNA-RRMR
Phr−3.0122.14E − 12Deoxyribodipyrimidine photolyaseDNA-RRMR
sbcD−2.0562.40E − 13Exonuclease SbcDDNA-RRMR
recG−2.1057.52E − 16ATP-dependent DNA helicase RecGDNA-RRMR; TRs
uvrC−2.6220Excinuclease ABC subunit CDNA-RRMR
uvrD−3.4120DNA helicase IIDNA-RRMR
ccmE−2.2977.41E − 10Cytochrome C-type biogenesis protein CcmEEM
ccmG−2.0013.24E − 06Cytochrome C biogenesis protein CcmGTPTMD; CHSPs; EM
PA1600−2.8192.31E − 06Probable cytochrome cEM
PA4571−2.7080Probable cytochrome cEM
PA4133−5.3440Cytochrome c oxidase subunit (cbb3-type)EM
ccoO1−2.4750Cytochrome c oxidase, cbb3-type, CcoO subunitEM
ccoQ1−2.2351.16E − 04Cytochrome c oxidase, cbb3-type, CcoQ subunitEM
nuoD−2.2160NADH dehydrogenase I chain C,DEM
nuoE−2.1629.15E − 13NADH dehydrogenase I chain EEM
narK1−2.5412.23E − 15Nitrite extrusion protein 1MPs; TSMs
narK2−5.6370Nitrite extrusion protein 2MPs; TSMs
narG−4.1570Respiratory nitrate reductase alpha chainEM
narJ−2.3860.014Respiratory nitrate reductase delta chainEM
narL−2.090Two-component response regulator NarLEM; TCRSs
Dnr−2.0658.03E − 14Transcriptional regulator DnrTRs