Research Article
Molecular Detection of Carbapenemase-Encoding Genes in Multidrug-Resistant Acinetobacter baumannii Clinical Isolates in South Africa
Table 2
List of primers used for PCR amplification of resistant genes.
| | Gene | Oligonucleotide sequence | Fragment size (bp) | Location | References |
| | 16SrRNA | F: 5′TTT AAG CGA GGA GGA GG3′
R: 5′ATT CTA CCA TCC TCT CCC3′ | 240 | 16SrRNA | [20] | | OXA-23-like | F: 5′GATCGGATTGGAGAACCAGA3′
R: 5′ATTTCTGACCGCATTTCCAT3′ | 501 | blaOXA-23 | [21] | | OXA-24-like | F: 5′GGTTAGTTG GCC CCC TTA AA3′
R: 5′AGTTGAGCGAAAAGGGGATT3′ | 246 | blaOXA-24 | [21] | | OXA-51-like | F: 5′TAATGCTTT GATCGG CCT TG3′
R: 5′TGGATTGCACTT CAT CTT GG3′ | 353 | blaOXA-51 | [21] | | OXA-58-like | F: 5′AAGTATTGGGGCTTGTGCTG-3′
R: 5′CCCCTCTGCGCTCTACATAC3′ | 599 | blaOXA-58 | [21] | | IMP-1 | F: 5′GATGGTATGGTGGCTCTTGT3′
R: 5′TTAATTTGCCGGACTTAGGC3′ | 448 | BlaIMP | [22] | | NDM-1 | F: 5′ATTAGCCGCTGCATTGAT3′
R: 5′CATGTCGAGATAGGAAGTG3′ | 154 | blaNDM | [23] | | VIM-like | F: 5′ACTCACCCCCATGGAGTTTT3′
R: 5′ACGACTGAGCGATTTGTGTG3′ | 815 | blaVIM | [24] | | SIM-1-like | F: 5′TAATGCTTT GATCGG CCT TG3′
R: 5′TGGATTGCACTT CAT CTT GG3′ | 353 | blaSIM | [21] | | AmpC | F: ACAGAGGAGCTAATCATGCG
R: GTTCTTTTAAACCATATACC | 1243 | blaAmpC | [25] | | ISAba1 | F: 5-CACGAATGCAGAAGTTG-3
R: 5-CGACGAATACTATGACAC-3 | 599 | ISAba1 | [18] | | intI1 | F: 5′CAG TGG ACA TAA GCC TGT TC3′
R: 5′CCC GAC GCA TAG ACT GTA3′ | 160 | Integrase gene | [19] |
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