Research Article

Prevalence of Methicillin-Resistant Staphylococcus aureus in Shrines

Table 10

Ingredients used in gram/liter in the preparation of M-Staphylococcus broth.

IngredientsGram/liter

Casein enzymic hydrolysate10.0
Yeast extract2.5
Lactose2.0
Mannitol10.0
Dipotassium hydrogen phosphate5.0
Sodium chloride75.0
Sodium azide0.049
PH (at 25°C)7.0 ± 0.2

About 104.55 grams of the media was suspended in 1000 ml distilled water. It was mixed thoroughly and heated to boiling for 5 minutes. The media were then autoclaved at 15 lbs at 121°C for 15 minutes.