Prevalence of E. coli ST131 among Uropathogenic E. coli Isolates from Iraqi Patients in Wasit Province, Iraq

Table 1

Components of 50 μl PCR master mix and amplification conditions for detection of UPEC phylogroups.

PCR reaction	Sterile DW (μl)	Primers	DNA (μl)	Amplification conditions
Quadruplex	37	8 μl: 1 μl each of; (i) chuA-1b and chuA-2; (ii) yiaA.1b and yiaA.2b; (iii) TspE4C2.1b and TspE4C2.2b; (iv) Acek.f and ArpA.r	5	(1) Initial denaturation at 94°C for 4 min; (2) 30 cycles of; (i) Denaturation at 94°C for 5 s; (ii) Annealing at 57°C (group E) or 59°C (quadruplex and group C) for 20 s; (iii) Extension at 72°C for 1 min; (3) Final extension at 72°C for 5 min
Group E	41	4 μl: 1 μl each of; (i) ArpAgpE.f and ArpAgpE.r; (ii) trpBA.f and trpBA.r	5
Group C	41	4 μl: 1 μl each of; (i) trpAgpC.1 and trpAgpC.2; (ii) trpBA.f and trpBA.r	5