Characterisation of Vibrio Species from Surface and Drinking Water Sources and Assessment of Biocontrol Potentials of Their Bacteriophages

Table 1

Oligonucleotide primers used for PCR amplification of Vibrio species-specific gene fragments.

Target organism	Primer sequence (5ʹ-3ʹ)	Targeted gene	Amplicon size (bp)	PCR cycling conditions	Reference
All bacterial strains (universal 16S rRNA gene sequence)	27F: AGAGTTTGATCATGGCTCAG; 1492R: GGTACCTTGTTACGACTT	16S rRNA	1420	Initial denaturation at 94°C for 3 minutes, 25 cycles of denaturation at 94°C for 1 minute, primer annealing at 55°C for 1 minute, elongation at 72°C for 2 minutes, and a final strand elongation step at 72°C for 10 minutes.	[37]
V. cholerae	F: CACCAAGAAGGTGACTTTATTGTG; R: GGTTTGTCGAATTAGCTTCACC	ompW	304	Initial denaturation at 94°C for 10 minutes, 30 cycles of denaturation at 94°C for 1 minute, primer annealing at 59°C for 1 minute, elongation at 72°C for 2 minutes, and a final strand elongation at 72°C for 10 minutes.	[38]
V. cholerae serogroup O1	F: TCTATGTGCTGCGATTGGTG; R: CCCCGAAAACCTAATGTGAG	rfbO1	638
V. cholerae	F: AAGACCTCAACTGGCGGTA; R: GAAGTGTTAGTGATCGCCAGAGT	sodB (1)	248	Initial denaturation at 95°C for 10 minutes, 35 cycles of denaturation at 92°C for 40 seconds, primer annealing at 57°C for 1 minute, elongation at 72°C for 1.5 minutes, and a final strand elongation at 72°C for 10 minutes.	[39]
V. parahaemolyticus	F: GCAGCTGATCAAAACGTTGAGT; R: ATTATCGATCGTGCCACTCAC	flaE	897
V. vulnificus	F: GTCTTAAAGCGGTTGCTGC; R: CGCTTCAAGTGCTGGTAGAAG	hsp60	410
V. mimicus	F: CATTCGGTTCTTTCGCTGAT; R:GAAGTGTTAGTGATTGCTAGAGAT	sodB	121
V. harveyi	F: CTTCACGCTTGATGGCTACTG; R: GTCACCCAATGCTACGACCT	vhh	235	Initial denaturation at 95°C for 10 minutes, 30 cycles of denaturation at 95°C for 1 minute, primer annealing at 50°C for 1 minute, elongation at 72°C for 1 minute, and a final strand elongation at 72°C for 10 minutes.	[40]