Characterisation of Vibrio Species from Surface and Drinking Water Sources and Assessment of Biocontrol Potentials of Their Bacteriophages

Table 2

Primer sequences and PCR amplification conditions for the detection of Vibrio virulent genes.

Target gene	Primer sequence (5ʹ-3ʹ)	Amplicon size (bp)	PCR cycling conditions	Reference
tdh	F: GTAAAGGTCTCTGACTTTTGGAC; R: TGGAATAGAACCTTCATCTTCACC	270	Initial denaturation at 94°C for 3 minutes, 30 cycles of denaturation at 94°C for 1 minute, primer annealing at 58°C for 1 minute, elongation at 72°C for 1 minute, and a final strand elongation at 72°C for 5 minutes.	[10]
trh	F: TTGGCTTCGATATTTTCAGTATCT; R: CATAACAAACATATGCCCATTTCCG	486
ctxAB	F: GCCGGGTTGTGGGAATGCTCCAAG; R: GCCATACTAATTGCGGCAATCGCATG	536	Initial denaturation at 94°C for 10 minutes, 30 cycles of denaturation at 94°C for 1 minute, primer annealing at 59°C for 1 minute, elongation at 72°C for 2 minutes, and a final strand elongation at 72°C for 10 minutes.	[41]
zot	F: TCGCTTAACGATGGCGCGTTTT; R: AACCCCGTTTCACTTCTACCCA	947
flrA	F: GAGGCAACAGCACCATCAAA; R: CGCATCTATATCAGGGACAA	503	Initial denaturation at 95°C for 5 minutes, 35 cycles of denaturation at 95°C for 45 seconds, primer annealing at 72°C for 45 seconds, and a final strand elongation at 72°C for 15 minutes.	[8]
vpsR	F: GGTGAGTAGCCATAAGCAAG; R: CATCCAGCACCACAGTATCT	911