Evaluation of 16S rRNA Hypervariable Regions for Bioweapon Species Detection by Massively Parallel Sequencing

Figure 4

Number of mapped reads for target bacterial species in a human DNA background sample sequenced in Ion Torrent PGM. Alignment was produced using the 16S rRNA consensus gene of the three species Escherichia coli, Salmonella enterica, and Vibrio cholera using Bowtie2. Different GE concentrations are in duplicate. 0 GE_I and II; 5 GE_I and II; 100 GE_I and II; 2,000 GE_I and II.