Evaluation of 16S rRNA Hypervariable Regions for Bioweapon Species Detection by Massively Parallel Sequencing

Table 1

Intraspecific and interspecific genetic similarity values of 16S rRNA gene.

Sequences extracted from the Greengenes database
#	Bacteria	Number of sequences		Average similarity ± standard deviation (%)						Consensus sequence (bp)
		Total	Filtered	Intraspecific			Interspecific
1	B. anthracis	234	17	99.56	±	0.6	75.81	±	2.58	1558
2	B. melitensis	90	12	99.60	±	0.5	82.55	±	7.81	1486
3	B. suis	16	4	99.50	±	0.7	80.84	±	7.18	1500
4	B. abortus	20	7	99.96	±	0.04	82.11	±	8.62	1496
5	B. mallei	67	14	99.98	±	0.04	79.39	±	5.8	1539
6	B. pseudomallei	132	31	99.69	±	0.51	79.52	±	5.77	1544
7	C. botulinum	224	28	94.48	±	4.84	74.23	±	5.07	1534
8	C. perfringens	480	57	99.72	±	0.2	74.97	±	4.57	1357
9	C. burnetii	19	11	99.72	±	0.2	80.89	±	2.82	1546
10	E. coli	9830	7	99.65	±	0.37	85.24	±	10.83	1543
11	F. tularensis	49	25	99.76	±	0.17	78.64	±	2.98	1536
12	R. prowazekii	7	7	99.25	±	1.22	75.64	±	3.39	1501
13	S. enterica	1025	47	99.43	±	0.43	85.16	±	10.29	1593
14	S. boydii	59	12	99.66	±	0.22	85.51	±	10.69	1545
15	S. dysenteriae	26	11	99.55	±	0.28	85.45	±	10.81	1551
16	S. flexneri	65	12	99.33	±	0.74	85.45	±	10.81	1552
17	S. sonnei	32	11	99.82	±	0.15	85.45	±	10.81	1547
18	V. cholerae	105	24	99.87	±	0.11	83.63	±	7.33	1549
19	Y. pestis	152	27	99.90	±	0.07	84.33	±	9.65	1592
	Total of sequences	12632	364