Antibacterial Potential of Secondary Metabolites from Indonesian Marine Bacterial Symbionts
Table 2
Morphological and biochemical characteristics of the marine bacteria isolates RC1–RC5 and RS1–RS7.
Characteristics
Isolate
RC1
RC2
RC3
RC4
RC5
RS1
RS2
RS3
RS4
RS5
RS6
RS7
Gram staining
+
+
+
+
+
+
+
+
+
+
+
+
Spore color
ISP1
White
White
White
White
White
White
White
White
White
White
White
White
ISP2
Grey
Grey
Pale yellow
Pale yellow
Grey
Olive
Grey
White
Greenish
Pale yellow
White
White
Pigment
ISP1
nd
nd
nd
d
nd
nd
nd
nd
nd
nd
nd
nd
ISP2
d
nd
nd
d
nd
d
nd
d
d
d
d
nd
Melanoid pigmentation
ISP1
—
—
—
—
—
—
—
—
—
—
—
—
ISP2
—
—
—
Brown
—
Black
—
Orange
Brown
Brown
—
—
NaCl tolerance
0% NaCl
+
+
+
+
+
+
+
+
+
+
+
+
5% (w/v) NaCl
+
+
+
+
+
+
+
+
+
+
+
+
7.5% (w/v) NaCl
+
+
+
+
+
+
+
+
+
−
−
±
10% (w/v) NaCl
+
+
±
+
+
+
+
−
+
−
−
−
12.5% (w/v) NaCl
+
+
−
−
−
+
−
−
+
−
−
−
15% (w/v) NaCl
−
−
−
−
−
−
−
−
−
−
−
−
17.5% (w/v) NaCl
−
−
−
−
−
−
−
−
−
−
−
−
pH tolerance
pH 4.0
−
−
−
−
−
−
−
−
−
−
−
−
pH 5.0
+
+
−
−
+
−
+
−
−
−
−
−
pH 8.0
+
+
+
+
+
+
+
+
+
+
+
+
pH 9.0
+
+
+
+
+
+
+
+
+
+
+
+
pH 10.0
+
+
+
+
+
+
+
+
+
+
+
+
pH 11.0
+
+
+
+
+
+
+
+
+
+
+
+
pH 12.0
+
+
+
+
+
+
+
+
+
+
+
+
pH 13.0
+
+
+
+
+
+
+
+
+
+
+
−
pH 13.80
−
−
−
−
−
−
−
−
−
−
−
−
Hydrolysis of
Protein
+
+
−
−
−
+
+
+
+
−
−
−
Starch
+
+
−
+
+
+
−
+
+
+
+
+
Utilization of carbohydrate
D-glucose
+
+
++
++
++
+
++
++
++
++
+
++
D-galactose
++
++
+
++
++
++
++
++
++
++
++
++
D-mannose
−
−
−
++
−
++
+
++
++
++
++
++
D-arabinose
++
++
−
++
++
++
++
+
++
++
+
+
D-xylose
+
++
−
+
+
++
++
++
++
++
++
+
D-fructose
++
++
++
++
++
++
++
++
++
++
++
+
Maltose
−
−
−
++
−
+
−
++
+
+
+
−
Dextrin
+
+
++
++
++
++
++
++
++
++
++
++
Inositol
++
++
−
+
+
+
++
+
+
+
++
−
Sucrose
−
−
−
−
+
+
+
+
+
+
+
−
Note. Sign for pigment tests: d, distinctive; nd, not distinctive. Sign for melanoid pigment: —, no melanoid pigment; sign for salt and pH tolerance: +, growth; −, no growth; sign for the carbohydrate utilization: ++, the isolate growth on tested carbon in the basal medium is equal to or greater than growth on the basal medium plus glucose; +, when the isolate growth on tested carbon in the basal medium was significantly better than that in the basal medium without carbon, but somewhat less than on glucose; —, when the isolate growth was similar to or less than growth in the basal medium without carbon.