Research Article
Antibacterial Potential of Secondary Metabolites from Indonesian Marine Bacterial Symbionts
Table 4
Antibacterial activities of EtOAc extracts prepared with different media.
| | Isolate | Medium | Dose, μg/well | Diameter of inhibition zone, mm | | SA | BS | SE | EC | PA | MS |
| | RC1 | MB + ASW | 200 | — | — | — | — | — | — | | ISP1 + ASW + TE | 200 | 8 | 10 | 10 | 8 | — | — | | AM | 200 | 10 | 14 | 14 | 10 | — | 4 |
| | RC2 | MB + ASW | 200 | 8 | 8 | 6 | 10 | — | 4 | | ISP1 + ASW + TE | 200 | 12 | 14 | 14 | 12 | — | 4 | | AM | 200 | — | 4 | 2 | — | — | 2 |
| | RC3 | MB + ASW | 200 | 12 | 20 | 14 | 14 | — | 6 | | ISP1 + ASW + TE | 200 | 12 | 18 | 18 | 12 | — | 14 | | AM | 200 | 16 | 22 | 14 | 10 | 4 | 16 |
| | RC4 | MB + ASW | 200 | 20 | 22 | 22 | 20 | 8 | 20 | | ISP1 + ASW + TE | 200 | 24 | 24 | 24 | 30 | 4 | 10 | | AM | 200 | 30 | 24 | 30 | 30 | 4 | 12 |
| | RC5 | MB + ASW | 200 | — | — | 8 | — | — | 2 | | ISP1 + ASW + TE | 200 | — | — | — | — | — | — | | AM | 200 | 10 | 10 | 8 | 10 | — | — |
| | RS1 | MB + ASW | 200 | 2 | 2 | 2 | 1 | — | 3 | | ISP1 + ASW + TE | 200 | 6 | 6 | 6 | 2 | — | — | | AM | 200 | 8 | — | 16 | — | | — |
| | RS2 | MB + ASW | 200 | 3 | 18 | 2 | 1 | — | 6 | | ISP1 + ASW + TE | 200 | 6 | 8 | 9 | 4 | — | — | | AM | 200 | 4 | 8 | 4 | 4 | 2 | 4 |
| | RS3 | MB + ASW | 200 | 22 | 20 | 22 | 24 | 4 | 4 | | ISP1 | 200 | 14 | 12 | 16 | 10 | — | 8 | | ISP1 + ASW | 200 | 20 | 20 | 21 | 20 | 6 | 14 | | ISP1 + ASW + TE | 200 | 10 | 10 | 8 | 12 | — | 1 | | AM | 200 | 2 | 16 | 22 | 24 | 1 | — |
| | RS4 | MB + ASW | 200 | 11 | 18 | 16 | 11 | — | 8 | | ISP1 | 200 | — | — | 2 | — | — | 1 | | ISP1 + ASW | 200 | 2 | — | — | — | — | — | | ISP 1ASW + TE | 200 | 6 | 12 | 12 | 8 | — | — | | AM | 200 | 1 | 4 | 3 | 2 | — | 4 |
| | RS5 | MB + ASW | 200 | 12 | 1 | 12 | 12 | — | 12 | | ISP1 | 200 | 2 | 1 | — | 1 | — | — | | ISP1 + ASW | 200 | 6 | 6 | 8 | 6 | — | — | | ISP1 + ASW + TE | 200 | — | — | — | — | — | — | | AM | 200 | 4 | 4 | 6 | 3 | — | 3 |
| | RS6 | MB + ASW | 200 | 4 | 4 | 4 | 4 | — | 2 | | ISP1 + ASW + TE | 200 | — | 1 | — | — | — | — | | AM | 200 | 8 | 12 | — | — | — | 2 |
| | RS7 | MB ASW | 200 | 5 | 6 | 2 | — | — | — | | ISP1 + ASW + TE | 200 | 8 | 12 | — | — | — | 6 | | AM | 200 | — | — | 2 | — | — | — |
| | Tetracycline (+control) | 2 | 22 | 20 | 20 | 22 | ND | ND |
| | Rifampicin (+control) | 8 | ND | ND | ND | ND | ND | 0.4 |
| | EtOAc (– control) | 20 μL | — | — | — | — | — | — |
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—, no inhibition zone; MB, modified Bennet; ASW, artificial seawater; TE, trace elements; AM, amylostatin medium; SA, Staphylococcus aureus; BS, Bacillus subtilis; SE, Salmonella enterica; EC, Escherichia coli; PA, Pseudomonas aeruginosa; MS, Mycobacterium smegmatis; ND, not determined.
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