Antibiotic Resistance and Virulence Gene Characteristics of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated from Healthy Edible Marine Fish

Table 1

Primer sequences used for the detection of antibiotic resistance genes.

Target gene	Oligonucleotide sequences (5′-3′)	Product size (bp)	Annealing temperature (°C)	References
ermA	F: TATCTTATCGTTGAGAAGGGATT	139	47.6
	R: CTACACTTGGCTTAGGATGAAA
ermB	F: CTATCTGATTGTTGAAGAAGGATT	142	47	[34]
	R: GTTTACTCTTGGTTTAGGATGAAA
ermC	F: CTTGTTGATCACGATAATTTCC	190
	R: ATCTTTTAGCAAACCCGTATTC
blaZ	F: ACTTCAACACCTGCTGCTTTC	173	49
	R: TGACCACTTTTATCAGCAACC
tetA	F: GCTACATCCTGCTTGCCTTC	209	50	[35]
	R: ATAGATCGCCGTGAAGAGG
tetM	F: AGTGGAGCGATTACAGAA	158	45	[36]
	R: CAT ATG TCC TGG CGT GTC TA
femA	F: AAAAAAGCACATAACAAGCG	132	45.5	[37]
	R: GATAAAGAAGAAACCAGCAG