A Comprehensive Study of the Relationship between the Production of β-Lactamase Enzymes and Iron/Siderophore Uptake Regulatory Genes in Clinical Isolates of Acinetobacter baumannii

Table 1

Oligonucleotide sequences used in this study and thermal cycling conditions.

Gene	Sequence of primers	Thermal cycles	Product size (bp)	Ref.
basD	F: CTCTTGCATGGCAACACCAC	95°C/5 min; (95°C/1 min, 57°C/60 sec, 72°C/45 sec) X30; 72°C/5 min	868	[2]
	R: CCAACGAGACCGCTTATGGT
bauA	F: TGGCAAGGTGAAAATGCACG	95°C/5 min; (95°C/1 min, 58°C/45 sec, 72°C/45 sec) X35; 72°C/5 min	224	[2]
	R: GCCGCATATGCCATCAACTG
pld	F: CCGTCAATTACGCCAAGCTG	95°C/5 min; (95°C/1 min, 57°C/1 min, 72°C/45 sec) X30; 72°C/5 min	662	[2]
	R: CTGACGCTACCTGACGGTTT
paaE	F: CTATTTAGGCGTTGCTGCGG	95°C/5 min; (95°C/1 min, 59°C/45 sec, 72°C/1 min) X30; 72°C/5 min	593	[2]
	R: CCTTACAACGACAGGTCGCA
feoB	F: AAGTCGCCAACTATGCCGGTGT	95°C/10 min; (95°C/1 min, 57°C/30 sec, 72°C/1 min) X30; 72°C/10 min	636	[3]
	R: AAGGCGCTGCCCATGCAAAAAC
hemO	F: TCGTGGCCGCTCAAAACAAGCA	95°C/5 min; (95°C/1 min, 59°C/1 min, 72°C/1 min) X30; 72°C/5 min	249	[3]
	R: AGGCCGCTAAATTACGTGCAGC
tonB	F: TTGTGGTGCCTCTGCAATCGGT	95°C/5 min; (95°C/1 min, 55°C/1 min, 72°C/1 min) X30; 72°C/5 min	1279	[3]
	R: TCGTGTACCCAAACGAGCAGGA