Evaluation of Cattle for Naturally Colonized Shiga Toxin-Producing Escherichia coli Requires Combinatorial Strategies

Table 1

Primers used in this study.

Sequence 5′⟶3″		Amplicon size (bp)	Reference
Wzx158-O26-F	GTA TCG CTG AAA TTA GAA GCG C	158	[57]
Wzx158-O26-R	AGT TGA AAC ACC CGT AAT GGC
Wzx72-O45-F	CGT TGT GCA TGG TGG CAT	72
Wzx72-O45-R	TGG CCA AAC CAA CTA TGA ACT
Wzx191-O103-F	TTG GAG CGT TAA CTG GAC CT	191
Wzx191-O103-R	ATA TTC GCT ATA TCT TCT TGC GGC
WbdI-O111-F	TGT TCC AGG TGG TAG GAT TCG	237
WbdI-O111-R	TCA CGA TGT TGA TCA TCT GGG
Wzx189-O121-F	AGG CGC TGT TTG GTC TCT TAG a	189
Wzx189-O121-R	GAA CCG AAA TGA TGG GTG CT
Wzx135-O145-F	AAA CTG GGA TTG GAC GTG G	135
Wzx135-O145-R	CCC AAA ACT TCT AGG CCC G
Stx (Stx1/2)-F1; Stx (Stx 1/2)-R1	TTT GTY ACT GTS ACA GCW GAA GCY TTA CG; CCC CAG TTC ARW GTR AGR TCM ACD TC	131 bp (stx1); 128 bp (stx2)
Eae-F	CAT TGA TCA GGA TTT TTC TGG TGA TA	102
Eae-R1	CTC ATG CGG AAA TAG CCG TTM
O26-F	CAATGGGCG GAAATTTTAGA	155	[56]
O26-R	ATAATTTTCTCTGCCGTCGC
O121	TCCAACAATTGTCGTGAAA	628
O121-R	AGAAAG TGTGAAATGCCCGT
Stx1-F	ATAAATCGCCATTCGTTGACTAC	180	[59]
Stx1-R	AGAACGCCCACTGAGATCATC
Stx2-F	GGCACTGTCTGAAACTGCTCC	255
Stx2-R	TCGCCAGTTATCTGACATTCTG
HlyA-F	GCATCATCAAGCGTACGTTCC	534
HlyA-R	AATGAGCCAAGCTGGTTAAGCT

¹Degenerate nucleotide codes are as follows: Y (C, T); S (C, G); W (A, T); R (A, G); M (A, C); D (A, G, T).