International Journal of Microbiology

Research Article

Evaluation of Cattle for Naturally Colonized Shiga Toxin-Producing Escherichia coli Requires Combinatorial Strategies

Table 2

Assay results per animal.
Animal numberNumber of mauve, fluorescent colonies shown as CFU/ml1Latex agglutination−based serogroup of non−O157 isolatesColony lysate PCRFecal−DNA PCRVero cell assay
O103O111O121O145O26O45eaestxstx1stx2hlyAO103O111O121O145O26O45eaeStxstx1stx2hlyACPE dilution3CPE scoresNo CPE4 with anti-Stx1 seraNo CPE with anti-Stx2 sera
5602−−−−−−−−−−−−−−−−−−−++−++81ny
56207 × 103O103+−−−−−+−−−++−−−−−−++++643yy
56575 × 104O103+−−−−−+−−−++−−−−−+++++81yy
NT2−−−−−−+−−−+
5724−−−−−−−−−−−−−−−−−−−+++++03yy
5743−−−−−−−−−−−−−−−−−−−+++++161ny
57591 × 103NT−−−−−−−−−−+−−−−−−−++++22yy
57601 × 108O103+−−−−−+−−−++−−−−−+++++41ny
57714 × 104NT−−−−−−+++++−−−−−−+++++21yy
57765 × 104NT−−−−−−+++−++−−−−−−++++41yy
5787−−−−−−−−−−−−−+−−−−−+++++41yn
5800−−−−−−−−−−−−−−−−−−−−++++81yy
58254 × 104O103+−−−−−+−−−++−−−−−++−++41ny
5845−−−−−−−−−−−−−+−−−−−++−++22ny
5868−−−−−−−−−−−−−+−−−−−+++++42yy
5874−−−−−−−−−−−−−−−−−−−+++++81yy
5875−−−−−−−−−−−−−−−−−−−+++++21yy
5889−−−−−−−−−−−−−−−−−−−+++++41yy
5901−−−−−−−−−−−−−+−−−−−+++++81yy
5915−−−−−−−−−−−−−+−−−−−++−++00n/an/a
59164 × 104O103+−−−−−+−−−++−−−−−+++++42yy
5922−−−−−−−−−−−−−−−−−−−−++++23yy
59352.4 × 104O103+−−−−−+−−−++−−−−−−++++03yy
O26−−−−+−+−−−+
59386.5 × 106O103+−−−−−+−−−++−−−−−+++++21yy
5939−−−−−−−−−−−−−−−−−−−+++++22yy
5949−−−−−−−−−−−−−−−−−−−−++++81yy
5950−−−−−−−−−−−−−−−−−−−+−+++83yn
5964−−−−−−−−−−−−−−−−−−−+++++83ny
5966−−−−−−−−−−−−−−−−−−−+++++81yy
59823.5 × 108O103+−−−−−+−−−++−−−−−++−++02ny
59853 × 108NT−−−−−−+−−−++−−−−−+++++81yy
5996−−−−−−−−−−−−−−−−−−−+++++41yy
6009−−−−−−−−−−−−−−−−−−−+++++43yn
6011−−−−−−−−−−−−−−−−−−−+++++81yy
6012−−−−−−−−−−−−−−−−−−−+++++21yy
6013−−−−−−−−−−−−−−−−−−−−++++02yy
60172 × 107O103+−−−−−+−−−++−−−−−+++++21yy
6044−−−−−−−−−−−−−−−−−−−+++++21nn
6049−−−−−−−−−−−−−−−−−−−+++++03yy
6069−−−−−−−−−−−−−+−−−−−+++++21nn
6082−−−−−−−−−−−−−−−−−−−−++++23yn
6089−−−−−−−−−−−−−−−−−−−+++++24yn
6101−−−−−−−−−−−−−−−−−−−+++++23yn
6120−−−−−−−−−−−−−+−−−−−+++++21yy
6122−−−−−−−−−−−−−−−−−−−+−−−+22yy
6130−−−−−−−−−−−−−−−−−−−+++++23yy
6137−−−−−−−−−−−−−−−−−−−−+++−42yy
6141−−−−−−−−−−−−−−−−−−−−++++22ny
61482 × 105NT−−−−−−+−−−+−−−−−−+++++23nn
6150−−−−−−−−−−−−−−−−−−−+++++22ny
6153−−−−−−−−−−−−−−−−−−−−+++−01ny
6171−−−−−−−−−−−−−+−−−−−+−+++03yy
6177−−−−−−−−−−−−−−−−−−−−++++43yy
6179−−−−−−−−−−−−−−−−−−−−+++−43yy
6507−−−−−−−−−−−−−−−−−−−−++++81ny
6509−−−−−−−−−−−−−−−−−−−−++++24yy
65295 × 104O26−−−−−−+−−−+−−−−−−−++++43ny
O121−−−−−−−−−−−
6542−−−−−−−−−−−−−+−−−−−−++++21yy
6561−−−−−−−−−−−−−−−−−−−+++++21ny
6582−−−−−−−−−−−−−+−−−−−+++++03yy
1Putative non-O157 STEC forming mauve, fluorescent colonies on CHROMagar STECTM, and postenrichment in EC broth were further analyzed by serology and colony PCR. 2NT, not typed to either of the “Big 6” non-O157 STEC serogroups by latex agglutination. 3The last dilution at which CPE still observed on the Vero cells was selected when setting up the toxin neutralization assays. 4y, yes; n, no; n/a, not applicable as no CPE was observed when at undiluted concentrations.