Research Article
Production, Optimization, and Characterization of an Acid Protease from a Filamentous Fungus by Solid-State Fermentation
Table 3
Macroscopic characteristics of potential fungal isolates.
| | Isolates | Media | Colony growth | Colony texture | CD, mean ± SEM | Obverse colony color | Reverse colony color | Degree of sporulation |
| | Z1BL1 | MEA | Medium | Floccose (cottony) | 28.3 ± 1.5 | Black | Dark black | High | | CDA | Low | Floccose (cottony) | 21 ± .5 | Dark white to gray | Pink | High | | PDA | High | Floccose (cottony) | 40.3 ± 1.2 | Dark black | Blue-black | High |
| | H1Gr1 | MEA | Medium | Rough | 30.7 ± 2.2 | Green | White gray | Low | | CDA | Medium | Rough | 29.6 ± .8 | White gray | Green | Low | | PDA | High | Rough | 43.0 ± 1 | Green | White to gray | Medium |
| | H1W1 | MEA | Medium | Floccose (cottony) | 31.3 ± 2 | White to brown | Brown | Low | | CDA | Low | Floccose (cottony) | 24.5 ± .6 | White to brown | Dark brown | Low | | PDA | High | Floccose (cottony) | 38.1 ± 1.1 | Yellow | White to yellow | Low |
| | SW2W1 | MEA | High | Floccose (cottony) | 40.4 ± 3 | Blue | Brown | Medium | | CDA | Low | Floccose (cottony) | 23.9 ± 5 | Brown | White to brown | Medium | | PDA | High | Floccose (cottony) | 45.0 ± .5 | Brown | Brown with white | High |
| | SW1WY1 | MEA | Medium | Rough | 25.5 ± 2.3 | White to yellow | White and brown | Medium | | CDA | Medium | Rough | 33.5 ± 1 | Yellow | Yellow at white | Medium | | PDA | High | Rough | 38.7 ± 1.2 | White yellow | Light yellow | High |
| | Z1BL3 | MEA | High | Floccose (cottony) | 41.6 ± 1.4 | Blue-black | Black | High | | CDA | Medium | Floccose (cottony) | 30.0 ± 2 | Black | Dark black | Medium | | PDA | High | Floccose (cottony) | 43.0 ± 1.5 | Dark black | Blue-black | High |
| | Z1Y2 | MEA | Medium | Rough | 29.4 ± .5 | Yellow | White | Low | | CDA | Very low | Rough | 13.7 ± 2.3 | Yellow | Brown | Low | | PDA | Medium | Rough | 31.5 ± 1.5 | White | White | Low |
| | H1BL1 | MEA | Low | Floccose (cottony) | 20.1 ± 1.9 | Blue-black | Dark black | Low | | CDA | High | Floccose (cottony) | 36.6 ± 1.4 | Black | Black | Low | | PDA | High | Floccose (cottony) | 40.3 ± .8 | Dark black | Blue-black | High |
| | Z1BL4 | MEA | High | Floccose (cottony) | 36.0 ± 2 | Blue-black | Black | Medium | | CDA | Low | Floccose (cottony) | 21.0 ± 1 | Black | Dark black | Low | | PDA | High | Floccose (cottony) | 36.2 ± 1.7 | Black | Blue-black | High |
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CD: colony diameter; MEA: malt extract agar; CDA: Czapek Dox agar; PDA: potato dextrose agar. Colony growth—Very low: <15 mm; Low: 15–25 mm; Medium: 25–35 mm, High: 35–45 mm; Very high: >45 mm [ 27]. |
