Overexpression of mfpA Gene Increases Ciprofloxacin Resistance in Mycobacterium smegmatis

Figure 2

RT-PCR of the mfpEA operon. (a) Lane 1: MWM 100 bp ladder (NEB); Lane 2: RT-PCR using primers 10-11; Lane 3: RT-PCR using primers 8-9; Lane 4: RT-PCR using primers 6-7; Lane 5: RT-PCR using the primers 4-5; and Lane 6: RT-PCR using primers 2-3. (b) Lane 1: MWM O′ Gene Ruler 1 kb DNA ladder (Thermo Scientific); Lane 2: No Reverse Transcriptase control (NRT) using primers 1–11; and Lane 3: RT-PCR using primers 1–11.