Overexpression of mfpA Gene Increases Ciprofloxacin Resistance in Mycobacterium smegmatis

Table 1

Strains and plasmids used in this work.

Strains and plasmids	Relevant characteristics	References
E. coli XL1-Blue	recA, endA, gyrA, thi-1, hsd R17, supE44, relA, lac [F′ proAB lacI^qZΔM15 Tn10 (Tet^R)].	Stratagene, CA, USA
M. smegmatis mc^2155	Mutant, transformable strain of M. smegmatis derived from the strain mc^26.	Snapper et al. [16]
M. smegmatis mc^2155 gyrA D94G	FQ-resistant mutant strain of M. smegmatis mc^2155 with the gyrA D94G substitution.	Our laboratory
pJEM15	Cloning and shuttle vector (9.5 kbp) with a promoterless lacZ gene. Kan^R (25 μg/ml).	Timm et al., [17]
pJEM15-5.11	pJEM15 cloning vector with the putative promoter region of the mfpEA operon.	This study
pALACE	Expression and shuttle vector (8221 bp) with a strong promoter (Pace) induced with acetamide. Hyg^R (50 μg/ml).	Lakshminarayan et al. [18]
pALACE-mfpA	pALACE vector with the mfpA gene of M. smegmatis.	This study