Characterization of Potential Virulence Factors of Vibrio mimicus Isolated from Fishery Products and Water

Table 2

Conditions used for the detection of genes.

Gene	vmhA	ctxA	tcpA	toxR	iut
Initial denaturation	95°C/5 min	95°C/5 min	95°C/5 min	95°C/5 min	95°C/5 min
Denaturation	95°C/45s	95°C/45s	95°C/45s	95°C/45s	95°C/45s
Annealing	58°C/45 s	55°C/30 s	57°C/40 s	51°C/45 s	62°C/1 min
Extension	72°C/45s	72°C/45s	72°C/45s	72°C/45s	72°C/1 min
Amplicon size (bp)	389	301	472	221	1573