The Antibacterial Activity of Crude Extracts of Secondary Metabolites from Bacterial Endophytes Associated with <i>Dicoma anomala</i>

Makuwa, Sephokoane Cindy; Serepa-Dlamini, Mahloro Hope

doi:https://doi.org/10.1155/2021/8812043

International Journal of Microbiology

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Abstract Introduction Materials and Methods Results and Discussion Conclusions Data Availability Disclosure Conflicts of Interest Acknowledgments References Copyright Related Articles

Research Article | Open Access

Volume 2021 | Article ID 8812043 | https://doi.org/10.1155/2021/8812043

The Antibacterial Activity of Crude Extracts of Secondary Metabolites from Bacterial Endophytes Associated with Dicoma anomala

Sephokoane Cindy Makuwa¹and Mahloro Hope Serepa-Dlamini¹

Academic Editor: Clemencia Chaves Lopez

Received27 May 2020

Revised28 Jan 2021

Accepted27 Mar 2021

Published13 Apr 2021

Abstract

Endophytic bacteria isolated from medicinal plants are recognized valuable sources of novel bioactive compounds with various activities such as antimicrobial, anticancer, and antiviral. In this study, eleven bacterial endophytes were isolated from surface sterilized roots and leave tissues, of medicinal plant Dicoma anomala. The bacterial endophytes were identified by sequencing the 16S rRNA gene, and belong to five genera viz Bacillus, Staphylococcus, Stenotrophomonas, Enterobacter, and Pantoea. The dominant genera were Bacillus with five strains, Staphylococcus with two strains, and Stenotrophomonas with two strains. The crude extracts of seven selected bacterial endophytes indicated antimicrobial activity against five pathogenic strains Escherichia coli (ATCC 25922), Bacillus cereus (ATCC 10876), Staphylococcus aureus (NCTC 6571), Pseudomonas aeruginosa (ATCC 27853), and Klebsiella oxytoca (ATCC 13182), with significant inhibition concentration ranging from 0.312 mg/ml to 0.625 mg/ml. Finally, based on the data analysis of the crude extracts of the endophytes, we identified bioactive secondary metabolites with reported biological activities such as antimicrobial, anti-inflammatory, and antioxidant properties with biotechnological applications in medicine, agriculture, and other industries. This study reported for the first time bacterial endophytes associated with D. anomala, with antimicrobial activity against bacterial pathogens.

1. Introduction

Medicinal plants are a source of biologically active compounds for novel drug development [1]. Historically, plants with healing properties were utilized in continents such as Asia and Africa, to treat diseases such as diarrhoea, headaches, and fevers [1]. In modern days, approximately 80% of the world’s population, especially within the developing countries, rely on herbal medicines from traditional healers to treat various diseases [2].

The use of medicinal plants for novel drug discovery is however a limiting factor, because at times high amounts of the plant materials are required for clinical studies before the products even make it to the market; moreover, some compounds are derived from endangered or endemic plant species. One of the advances made in addressing these concerns was the discovery of microorganisms called endophytes living within plants, which produce the same or similar compounds like those produced by their plant hosts [1, 3]. Endophytes are either fungi or bacteria and reside inside the plant tissues without causing any harm. Of interest in the current study are bacterial endophytes. Endophytic bacteria belong to various genera and many of the biological active substances extracted from endophytic bacteria are reported to be novel with various bioactivities [4]. Microbes are a good source for extraction of biologically active compounds due to their ease of isolation, growth, and inability to impact negatively on the environment and agricultural productivity [5, 6]. From a commercial and industrial point of view, it is easier to expand microbial fermentation which can ultimately increase production of biologically active compounds. Thus, microbes associated with medicinal plants present an opportunity for isolating novel biologically active compounds, with benefit of preserving medicinal plants, as minimal plant material is required for endophyte isolation.

Endophytes significantly ameliorate plant growth by a number of mechanisms including increase of uptake of essential nutrients such as phosphate, ammonia, and nitrogen, producing phytohormones such as indole-3-acetic acid and gibberellin, and inhibiting pathogen proliferation through the production of secondary metabolites with antimicrobial activity [1]. Moreover, many antimicrobial compounds produced by bacterial endophytes belong to several structural classes such as peptides, alkaloids, steroids, quinines, terpenoids, phenols, and flavonoids with various applications [7]. Therefore, there is a worldwide renewed scientific effort to isolate endophytes and study their natural products, which play a vital role as antibacterial, antiviral, antioxidant, antiarthritic, and antidiabetic and as immune-suppressive compound.

Dicoma anomala is a perennial herb with an underground tuber; it belongs to the family Asteraceae. Dicoma anomala is mostly distributed in southern African countries; in South Africa it is found in Limpopo, North-West, Gauteng, Mpumalanga, Free State, Northern Cape, and KwaZulu-Natal provinces [8, 9]. Dicoma anomala roots and leaves have ethnomedicinal uses for ailments such as colds and coughs, fevers, ulcers, labour pains, dysentery, stomach problems, dermatosis, sores, and wounds [8]. Literature indicates that D. anomala is known to produce secondary metabolites including acetylenic compounds, diterpene, flavonoids, phenolic acids, phytosterols, saponins, sesquiterpene lactones, tannins, and triterpenes [10] and a study reported by [8] has shown that these compounds possess antimicrobial, anti-inflammatory, antioxidant, antiplasmodial, anticancer, and cytotoxicity, antihyperglycaemic, and hepatoprotective properties. Consequently, extracting and identifying novel secondary bioactive compounds from bacterial endophytes associated with this plant could become the alternative option to overcome levels of drug resistance and conserve the plant species eventually.

This study was undertaken to isolate and identify bacterial endophytes from D. anomala, which is a less explored medicinal plant with ethno-botanical history. In addition, the selected endophyte’s secondary metabolite crude extracts were assayed for antibacterial activity and further identification of the secreted secondary metabolites using Gas Chromatography High-Resolution Time-of-Flight Mass Spectrometry (GC-HRTOF-MS). To the best of our knowledge, there has been no previous work published on antimicrobial activity of bacterial endophytes isolated from medicinal plant D. anomala.

2. Materials and Methods

2.1. Plant Sample Collection and Identification

Dicoma anomala plant material was collected in April 2018 from the natural habitat with slit soil type in Eisleben, Limpopo province, South Africa (23.52 S 29.824 E). Whole plants including roots were placed in sterile polyethylene bags and transported to the laboratory under 4°C. The identification of the plant material was carried out at the University of Johannesburg Herbarium (JRAU) and a sample specimen of the plant material was deposited JRAU with voucher specimen number Serepa-Dlamini 204 and species name Dicoma anomala. The remaining collected plant material was immediately processed in the laboratory.

2.2. Isolation of Endophytic Bacteria

Immediately after collection, in the lab, endophytic bacteria were isolated from fresh leaves and roots of the plant following the procedure described by [11]. In brief, plant parts were washed thoroughly with running tap water and surface sterilized with 70% ethanol for 5 minutes, followed by a rinse with sterile distilled water, soaking in 2% NaClO for 3 minutes, and final rinse with sterile distilled water 3 times and the final wash water was plated on to nutrient agar plates as control. The surface-disinfected plant parts were crushed using sterile mortar and pestle and macerated with phosphate buffer (8 g NaCl, 0.2 g KCl, 1.44 g Na₂HPO₄, and KH₂PO₄ at pH 7.4). Following sterile techniques, the homogenate was streaked on nutrient agar plates and incubated together with the control plates at 28°C for 2–7 days. The plates were observed for growth daily; grown colonies were subcultured several times until pure single colonies were obtained. Confirmation of culture purity of the strains was selected on the basis of phenotypic characteristics such as colony morphology, colony colour, colony size, and Gram reaction. In total, 11 isolates were selected for this study and preserved in 30% glycerol stock (v/v) solution for long-term storage at −80°C.

2.3. Genotypic Characterization of the Bacterial Endophytes

2.3.1. Genomic DNA Extraction

Genomic DNA was extracted from pure solid colonies using the Zymo Research Fungal/Bacterial DNA MiniPrep Kit as per manufacturer’s protocol (Zymo Research, USA). The extracted DNA was electrophoresed on 1% agarose gel and the concentration was determined using the Nanodrop Spectrophotometer (Thermo Fisher Scientific, USA).

2.3.2. 16S rRNA Gene Sequence Analysis

Using Polymerase chain reaction (PCR), DNA of each bacterial isolate was amplified using 16S rRNA gene universal primers described by [12]. The amplification of the 16S rDNA was carried out in a reaction tube with a final volume of 25 μL containing 2.5 μL (<1,000 ng) of template DNA, 2.5 μL (10 μM) of each forward primer 5′-AGAGTTTGATCCTGGCTCAG-3′F and reverse primer 5′-AAGGAGGTGATCCAAGCCGCA-3′ R, 4 μL of 2X PCR Master mix with standard buffer (20 mM Tris-HCI, 1.8 mM MgCl₂, 22 mM NH₄Cl, 22 mM KCl, 0.2 mM dNTPs, 5% glycerol, 0.06% IGEPAL® CA-630, 0.05% Tween® 20, 25 units/mL One Taq® DNA polymerase) and final volume was filled up 25 μL with nuclease-free water. A negative control (PCR mix without DNA) was included in all PCR experiments. Using MyCycler™ Thermal Cycler (Bio-Rad, USA), the PCR reaction conditions were as follows: initial denaturation at 94°C for 3 minutes, followed by 35 cycles of denaturation at 94°C for 1 minute, annealing at 48°C for 1 minute and extension at 72°C for 2 minutes, and a final extension of 72°C for 10 minutes. The PCR products were purified with ExoSAP-it™ (Thermo Fisher Scientific, USA) and sent for sequencing at a commercial service provider Inqaba Biotechnical Industries (Pty) Ltd, Pretoria, South Africa.

2.3.3. Phylogenetic Analysis

Raw sequence data were used to create consensus sequences using BioEdit Sequence Alignment Editor version 7.2.6 [13]. The obtained 16S rDNA sequences were subjected to Basic Local Alignment Search Tool (BLAST) analysis against the rRNA sequence database (Bacteria and Archaea) at the National Centre for Biotechnology Information (NCBI) (available at http://blast.ncbi.nlm.nih.gov) to compare with the closest related bacterial species. Only bacterial species with highest similarity identity percentage (90–100%) were selected for phylogenetic analysis. Phylogenetic trees were constructed using MEGA 7 [14] after alignment with MUSCLE [15]. DNA substitutions were done according to the Tamura Nei model. Phylogenetic relatedness of the endophytes with other close relatives was clustered using maximum-likelihood method. Bootstrap replications of 1000 were used as the statistical confidence of the nodes in the phylogenetic trees. Outgroups were considered to support differences among species. The nucleotide sequences of 16S rRNA genes were deposited to GenBank ((https://www.ncbi.nlm.nih.gov/genbank/) and assigned accession numbers.

2.3.4. Nucleotide Sequence Accession Numbers

The bacterial isolates were assigned the following accession numbers: MN029049 (Stenotrophomonas sp. strain MHSD20), MN029050 (Enterobacter sp. strain MHSD22), MN029051 (Staphylococcus sp. strain MHSD24), MN029052 (Staphylococcus sp. strain MHSD26), MN029053 (Bacillus sp. strain MHSD28), MN078165 Bacillus sp. strain MHSD13, MN078166 (Bacillus sp. strain MHSD14), MN078167 (Bacillus sp. strain MHSD16), MN078168 (Bacillus sp. strain MHSD17), and MN093331 (Pantoea sp. strain MHSD15).

2.4. Antimicrobial Assay Minimum Inhibitory Concentration (MIC)

2.4.1. Preparation of Bacterial Endophyte’s Secondary Metabolite Crude Extracts

Secondary metabolites were extracted from selected isolated bacterial endophytes; care was taken to include species which were not assayed previously. The selected species included Stenotrophomonas sp. strain MHSD20, Enterobacter sp. strain MHSD22, Staphylococcus sp. strain MHSD26, Bacillus sp. strain MHSD28, Stenotrophomonas sp. strain MHSD12, Bacillus sp. strain MHSD14, and Pantoea sp. strain MHSD15. The bacterial endophytes were cultured in 1L Luria Broth (LB) for 7 days at 28°C on a shaking incubator at 180 rpm. After cultivation, 2 g/L of sterilized Amberlite® XAD-7-HP resin (Sigma-Aldrich, Darmstadt, Germany) was added to the cultures and shaken for 2 hours at 180 rpm, to absorb the secondary metabolites. The resin was filtered through a cheesecloth and eluted 3 times with 200 mL of acetone. The acetone was evaporated using rotary evaporator. The remaining liquid containing the crude extracts was further extracted 3 times with ethyl acetate in a 1 : 1 ratio (v/v) and the ethyl acetate was evaporated and concentrated with rotary evaporator. The crude extracts containing the secondary metabolites were transferred to 20 mL beakers and covered with foil and stored at room temperature to dry out until further analysis.

2.4.2. Minimum Inhibitory Concentration (MIC)

Minimum inhibitory concentration studies of the bacterial endophyte’s crude extracts were performed according to the method by [16] with some modifications. Briefly, stock solutions of the extracts were prepared by dissolving 0.02 g in 1 mL dimethyl sulfoxide (DMSO) to produce a final concentration of 20 mg/mL, which were then serially diluted to concentrations of 10 mg/mL, 5 mg/mL, 2.5 mg/mL, 1.25 mg/mL, 0.625 mg/mL, and 0.312 mg/mL using Mueller-Hinton broth. The pathogenic test strains used were Gram-negative bacteria Escherichia coli (ATCC25922), Pseudomonas aeruginosa (ATCC27853), and Klebsiella oxytoca (ATCC13182) and Gram-positive bacteria Staphylococcus aureus (NCTC6571) and Bacillus cereus (ATCC10876). Using McFarland 0.5 standard, 50 μL of each pathogen was inoculated in 15 mL of Muller-Hinton broth and incubated at 37°C for 24 hours. Following aseptic techniques in 96 well microtitre plates, 100 μL of pathogenic test strains was added horizontally and 100 μL of the diluted crude extract concentrations was added vertically starting from high to low concentrations. Negative controls [100 μL DMSO and 100 μL sterile Mueller-Hinton broth, 1 : 1 ratio (v/v)] and positive control 1 mg/mL Streptomycin antibiotic (Sigma-Aldrich, Switzerland) were added vertically in the wells subsequent to those with low concentrations. The microtitre plates were incubated at 37°C for 24 hours. The MIC assays were conducted in triplicates. After incubation, ten microliters of resazurin sodium salt solution [0.02% (w/v)] was added to the wells as an indicator of microbial growth and incubated for another 2 hours. The ability of the crude extracts to inhibit pathogenic strains was indicated by no colour change of resazurin sodium salt solution (remains blue) and the colour change from blue to pink after incubation indicated no bacterial inhibition. The MIC (lowest concentration of each extract displaying no visible growth) values were visually determined.

2.5. Metabolite Fingerprinting Analysis Using GC-HTOF-MS

Metabolite profiling of the crude extracts was performed at a two-dimensional mode using Pegasus GC-HRTOF-MS system (Leco Corporation St. Joseph, MI, USA). One microliter of each sample was injected and helium was used as the carrier gas at 1 mL/min flow rate. The primary column was a Restek Rtx-5siLMS (30 m × 250 μm d.f.) and the secondary column was Restek Rxil7siLMS (1 m × 250 μm × 0.25 μm d.f.). The GC oven temperature was maintained at 60°C for 1 minute, then ramped to 330°C at 10°C/min, and thereafter held for 5 minutes at transfer line temperature of 280°C. The inlet temperature was 250°C. The MS was optimized at −70 eV (electron energy) with an ion source at 250°C. The collected mass range was 40 to 660 m/z with an acquisition rate of 10 spectra/second. The generated data was analysed using ChromaTOF® software. The NCBI Pubchem database, available at https://pubchem.ncbi.nlm.nih.gov/ [17], was used to interpret functional groups and PASS online database, available at http://www.way2drug.com/passonline, was used to predict the biological activities of the metabolites [18].

2.6. Statistical Analysis

The MIC of the crude’s extracts was statistically validated by one-way analysis of variance (ANOVA) using in Microsoft Excel 2016. Significant differences at ≤ 0.05 were considered statistically different.

3. Results and Discussion

3.1. Morphological Identification

In this study, a total of 11 bacterial endophytes from 5 genera belonging to two phyla (Firmicutes and Proteobacteria) were isolated from surface sterilized leaves and roots of a healthy medicinal plant D. anomala. Majority of the endophytic bacteria were isolated from the roots with 6 isolates, followed by the leaves with 5 isolates (Table 1). A great morphological diversity was observed where each isolate showed unique characteristics in terms of colony colour, shape, size, and margins. The isolates were grouped into two distinct classes based on Gram staining technique, The Gram-positive bacteria (7 isolates) and the Gram-negative bacteria (4 isolates).

The surface sterilization technique was adequate as the control plates had shown no microbial growth. Therefore, bacterial colonies that grew on the media were regarded as endophytic bacteria of D. anomala. To the best of our knowledge, this is the first report on isolation of bacterial endophytes from D. anomala. Bacillus genus occurred in both leaves and root samples, having a larger occurrence with 5 strains. These results are comparable to various studies that have reported different genera of Firmicutes and Proteobacteria which included Bacillus [19], Staphylococcus [20], Stenotrophomonas [21], Enterobacter [22], and Pantoea [23]; bacterial species from these genera seem to be occurring as bacterial endophyte communities of many plants.

The distribution and diversity of bacterial endophytes in plants are influenced by many factors including host plant species, age and type of the plant tissue, geographical and habitat distribution, sampling season, surface sterilization method, growth media, and culture conditions [24]. In this study, the plant material was collected from a slit soil in April 2018, we utilized nutrient agar for growth and isolation of the bacterial endophytes, and as such this resulted in limited number of identified bacterial endophytes.

3.2. Molecular Identification and Phylogenetic Analysis of the Bacterial Endophytes

The 16S rRNA gene sequence-based identification is a rapid method for molecular identification of bacterial species. Firmicutes and Proteobacteria were found among the isolated endophytic bacteria (Table 2). The bacterial isolates were classified into five genera which include Bacillus with 5 five isolates, Staphylococcus with 2 isolates, Stenotrophomonas with 1 isolate, Enterobacter with 1 isolate, and Pantoea with 1 isolate, indicating Bacillus as the most dominant genus. The 16S rDNA sequences obtained were submitted to NCBI and assigned the accession numbers provided in Table 2. Each genus was analysed in separate phylogenetic trees (Figures 1–5).

Staphylococcus sp. strain MHSD24 and MHSD26 sequences were aligned with strains of Staphylococcus genus, and Escherichia coli AE-1 (AB269763) was used as an outgroup (Figure 1). Strain MHSD24 was closely related to Staphylococcus warneri MK791673 with 98.29% similarity whilst strain MHSD26 was closest to Staphylococcus pasteuri MK875469 with 97.44% similarity (Table 2). Phylogenetic analysis shows that strain MHSD26 had a polyphyletic cluster with Staphylococcus pasteuri DCo1 and S. warneri BPB17, and strain MHSD24 also had a polyphyletic relationship with S. warneri PSB16. Numbers above or below the nodes indicate bootstrap values generated after 1000 replications.

Enterobacter sp. strain MHSD22 was closely related to Enterobacter ludwigii KU054383 with 97.48% similarity (Table 2) and phylogenetic analysis showed strain MHSD22 had polyphyletic relationship with closely related Enterobacter species; Streptococcus mitis (AY360354) was used as an outgroup (Figure 2).

Stenotrophomonas strain MHSD20 and MHSD12 were aligned with closely related species of Stenotrophomonas genus, and Escherichia coli AE-1 (AB269763) was used as an outgroup taxon (Figure 3). Strain MHSD20 was closely related to Stenotrophomonas maltophilia (KM279660) with 98.55% similarity and strain MHSD12 was closely related to Stenotrophomonas maltophilia (MK734043) with 96.85% similarity (Table 2). Phylogenetically, both strains showed high similarity among themselves, having a polyphyletic relationship with closely related Stenotrophomonas species (Figure 3). Pantoea sp. strain MHSD15 had a polyphyletic relationship with closely related species (Figure 4) and it had a 92.70% similarity with Pantoea sp. (MH769026) (Table 2).

Similarly, the sequences from that of Bacillus sp. strain MHSD28 were closely related to Bacillus cereus (MK503979) with 97.88% similarity, Bacillus sp. strain MHSD13 was closely related to Bacillus infantis (MK850860) with 97.33% similarity, Bacillus sp. strain MHSD14 was closely related to Bacillus thuringiensis (LT838181) with 97.68% similarity, Bacillus sp. strain MHSD16 was closely related to Bacillus sp. (EUC01244) with 90.96% similarity, and Bacillus sp. strain MHSD17 was closely related to Bacillus cereus (KJ935725) with 98.15% similarity as indicated in Table 2. Phylogenetic analysis showed sister relationship between strain MHSD28 and MHSD16, strain MHSD17 and MHSD14 formed a polyphyletic relationship with related Bacillus species, and MHSD13 also had a polyphyletic relationship with closely related species (Figure 5).

The isolated bacterial endophytes were identified to genus level using the 16S rRNA gene phylogenetic analysis; we suggest sequencing other genes other than the 16S rRNA for further species description and phylogenetic delineation. The 16S rRNA gene has been recommended for identification of bacteria and this technique is regarded as the gold standard for species classification because the gene is ubiquitous in prokaryotes, containing approximately 1500 bases which are adequate for species analysis [25]. The 16S RNA genes also consist of variable regions that enable comparison of species between distantly related species and 16S rRNA gene is less likely to undergo horizontal gene transfer (HGT) [42]. Although the 16S rRNA gene is suitable for bacterial identification [26], many bacterial species cannot be differentiated by their 16S rRNA gene sequences as it gives high similarity between closely related species which is also indicated by the formation of polyphyletic relationships on a phylogenetic tree [25]. Bacterial endophytes identified in this study formed polyphyletic relationship with closely related strains. In a separate study [27], based on phylogenomic analysis, it was indicated that Stenotrophomonas strain MHSD12 is closely related to Stenotrophomonas pavanii strain DSM 25135 which is an endophyte isolated from sugarcane [28].

3.3. Antibacterial Assay Using Minimum Inhibition Concentration

The antibacterial activity of endophytic bacteria’s crude extracts was determined against five pathogenic test strains: Gram-negative bacteria Escherichia coli (ATCC25922), Pseudomonas aeruginosa (ATCC27853), and Klebsiella oxytoca (ATCC13182); Gram-positive bacteria Staphylococcus aureus (NCTC6571) and Bacillus cereus (ATCC10876). The MIC activity varied depending on the test strain used, most extracts showed interesting results, and the positive control Streptomycin at 1 mg/mL was effective against all the test strains. Minimum inhibition concentration of the extracted crude extracts from bacterial endophyte ranged from 0.312 mg/mL to 10 mg/mL. The lowest MIC value was 0.312 mg/mL and other crude extracts indicated MIC values higher than 1 mg/mL as shown in Table 3.

Bacterial endophyte Stenotrophomonas sp. strain MHSD20 had MIC values ranging from 0.625 mg/mL to 5 mg/mL, inhibiting B. cereus at 0.625 mg/mL. Enterobacter sp. strain MHSD22 showed MIC values from 1.25 mg/mL to 10 mg/mL which were considered noninhibiting. The crude extract of Staphylococcus sp. strain MHSD26 had MIC concentration values ranging from 0.312 mg/mL to 10 mg/mL, inhibiting B. cereus and S. aureus at 0.625 and 0.312 mg/mL, respectively. Bacillus sp. strain MHSD28 showed MIC values ranging from 0.312 mg/mL to 10 mg/mL, inhibiting S. aureus 0.312 mg/mL and E. coli at 0.625 mg/mL. Crude extracts of Bacillus sp. strain MHSD14 had MIC values of 0.312 mg/mL to 5 mg/mL inhibiting S. aureus at 0.312 mg/mL and finally Pantoea sp. strain MHSD15 crude extracts had MIC values of 0.625 mg/mL to 10 mg/mL inhibiting S. aureus at 0.625 mg/mL. These findings are congruent with several studies, which revealed that endophytic bacteria have proven to be potential reliable sources of novel bioactive compounds with antimicrobial activity [10]. The MIC values of crude extracts <1 mg/mL are classified to have significant antibacterial activity while extracts with MIC values > 1 mg/mL are classified as noninhibitors [29]. Most of the endophyte’s crude extracts had significant inhibition values with the lowest MIC values 0.625 mg/mL and 0.312 mg/mL. Enterobacter sp. strain MHSD22 did not have significant inhibition against all the test strains. The MIC values of the crude extracts at 0.625–0.325 mg/mL were significantly different ( < 0.05), with the exception of Enterobacter sp. strain MHSD22 which had MIC values higher than 1 against all test strains.

The difference in the inhibition between Gram-positive and Gram-negative may be due to variation in their cell wall structure. Gram-negative bacteria have an extra outer membrane, which could increase impermeability to antimicrobials. Similar results were observed in other studies [30]. The antibacterial activities of crude extracts of the bacterial endophytes obtained in this study provide evidence that endophytic bacteria could have potential in outcompeting pathogenic bacteria and this necessitates their use in drug discovery.

3.4. Gas Chromatography High-Resolution Time-of-Flight Mass Spectrometry Analysis

Bacterial endophytes are reported to produce same or similar bioactive compounds produced by the host plant [1], thus making them a promising source of novel molecules with various biotechnological applications. Dicoma anomala has ethnomedicinal history for the treatment of coughs, cold fever, and labour pains with pharmacological potential to antibacterial, anti-inflammatory, and antiplasmodial activities that could be a potential target for bioactive secondary metabolites studies [8]. Different classes of secondary metabolites such as phenolic acids, flavonoids, and triterpenes had previously been identified from D. anomala crude extracts [8]. In the current study, we identified 15 compounds from ethyl acetate crude extracts of bacterial endophytes isolated from D. anomala. Identification and characterization of secondary metabolites can lead to discovery of new compounds for drug development [31] including discovery of compounds that are of interest in food, cosmetic, and other industries. Most of the identified compounds in this study have been reported to possess interesting biological activities ranging from antimicrobial, antioxidant, and anti-inflammatory as summarized in Table 4. Benzyl benzoate is reported to function as fragrance ingredient, pesticides, pH adjusters, preservatives, solvents, and/or viscosity decreasing agents in cosmetic products [32]. Benzyl benzoate was identified in both Enterobacter sp. strain MHSD22 and Stenotrophomonas sp. strain MHSD12 bacterial endophytes extracts and it is reported to have antibacterial activity [33]. The identification of this compound indicates that, in addition to discovery of drug developments, bacterial endophytes are also useful for discovery of other bioactive compounds beneficial in food, cosmetic, and chemical industry [34]; this discovery necessitates further studies.

9-Octadecenamide, (Z)- is an oleamide, an amide derived oleic acid biosynthesis that has shown antioxidative and hypolipidemic bioactivities [35]. It was previously identified from Bacillus crude extracts isolated from the rhizosphere of groundnut [36]. According to Cheng et al. [35], this compound was also reported as a potential medicinal treatment for mood and sleeping disorders; it was identified in Stenotrophomonas sp. strain MHSD12 and Pantoea sp. strain MHSD15 bacterial endophytes extracts. Hexadecane was identified in all bacterial endophyte extracts with Stenotrophomonas sp. strain MHSD20 as an exception. It is known to possess antifungal and antibacterial as well as antioxidant activity [35, 37]. Pyrrolo[1,2-a] pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl)- was identified in Staphylococcus sp. strain MHSD26, Enterobacter sp. strain MHSD22, and Bacillus sp. strain MHSD28 extracts and this compound has been reported as an antibiotic, anti-inflammatory, cholesterol reducing, and antitumor drug agent [38].

Heptacosane has been reported to have antioxidant properties [39] and was previously identified from Pseudomonas spp. isolated from Vigna radiate [40]. In the current study, heptacosane was identified from Bacillus sp. strain MHSD28, Bacillus sp. strain MHSD14, Stenotrophomonas sp. strain MHSD12, Staphylococcus sp. strain MHSD26, and Pantoea sp. strain MHSD15 extracts. Most of the compounds identified in this study have antimicrobial activity, which explains the significant antibacterial activity of the crude extracts. To our knowledge, this is the first study to report for the first time bacterial endophytes associated with D. anomala, with antimicrobial activity against bacterial pathogens and non-targeted profiling of their secondary metabolites.

4. Conclusions

Based on the results, we conclude that D. anomala does harbor diverse types of endophytic bacteria. Moreover, antimicrobial activity of these endophytes’ crude extracts was significant against pathogenic strains. Further investigation should be conducted to isolate biologically active compounds from bacterial endophytes with antimicrobial activity for drug development and use in other industries such as food and cosmetics.

Data Availability

The 16S rRNA gene data of this study are available from the corresponding author upon request. The reported bacterial endophytes in this study have been deposited in GenBank with the following accession numbers: MN029049, MN029050, MN029051, MN029052, MN029053, MN078164, MN078165, MN078166, MN093331, MN078167, and MN078168.

Disclosure

This study is based on the M.Sc. dissertation work of Ms. SC Makuwa.

Conflicts of Interest

The authors declare that they have no conflicts of interest regarding the publication of this paper.

Acknowledgments

Thanks are due to South African National Research Foundation, Thuthuka (grant no. TTK170405225920), and University of Johannesburg, Faculty of Science, for the financial support for the study. Ms. SC Makuwa received DAAD-NRF joint in country scholarship (grant number: SFH180523334030) and University of Johannesburg, Faculty of Science Merit bursary.

References

A. Alvin, K. I. Miller, and B. A. Neilan, “Exploring the potential of endophytes from medicinal plants as sources of antimycobacterial compounds,” Microbiological Research, vol. 169, no. 7-8, pp. 483–495, 2014.
View at: Publisher Site | Google Scholar
A. Gurib-Fakim, “Medicinal plants: traditions of yesterday and drugs of tomorrow,” Molecular Aspects of Medicine, vol. 27, no. 1, pp. 1–93, 2006.
View at: Publisher Site | Google Scholar
B. Jasim, C. John Jimtha, M. Jyothis, and E. K. Radhakrishnan, “Plant growth promoting potential of endophytic bacteria isolated from Piper nigrum,” Plant Growth Regulation, vol. 71, no. 1, pp. 1–11, 2013.
View at: Publisher Site | Google Scholar
P. Golinska, M. Wypij, G. Agarkar, D. Rathod, H. Dahm, and M. Rai, “Endophytic actinobacteria of medicinal plants: diversity and bioactivity,” Antonie van Leeuwenhoek, vol. 108, no. 2, pp. 267–289, 2015.
View at: Publisher Site | Google Scholar
J. E. John, “Natural products as lead-structures: a role for biotechnology,” Drug Discovery Today, vol. 15, pp. 11-12, 2010.
View at: Publisher Site | Google Scholar
D. J. Newman and G. M. Cragg, “Natural products as sources of new drugs over the 30 years from 1981 to 2010,” Journal of Natural Products, vol. 75, no. 3, pp. 311–335, 2012.
View at: Publisher Site | Google Scholar
H. Yu, L. Zhang, L. Li et al., “Recent developments and future prospects of antimicrobial metabolites produced by endophytes,” Microbiological Research, vol. 165, no. 6, pp. 437–449, 2010.
View at: Publisher Site | Google Scholar
J. V. M. Becker, M. M. van Der Merwe, A. C. van Brummelen et al., “In vitro anti-plasmodial activity of Dicoma anomala Subsp. gerrardii (Asteraceae): identification of its main active constituent, structure-activity relationship studies and gene expression profiling,” Malaria Journal, vol. 10, Article ID 295, 2011.
View at: Publisher Site | Google Scholar
South African National Biodiversity Institute (SANBI), 2019, http://pza.sanbi.org/dicoma-anomala.
A. Maroyi, “Dicoma anomala sond.: a review of its botany, ethnomedicine, phytochemistry and pharmacology,” Asian Journal of Pharmaceutical and Clinical Research, vol. 11, no. 6, pp. 70–77, 2018.
View at: Publisher Site | Google Scholar
P. N. Patle, N. P. Navnage, and P. R. Ramteke, “Endophytes in plant system: roles in growth promotion, mechanism and their potentiality in achieving agriculture sustainability,” International Journal of Chemical Studies, vol. 6, pp. 270–274, 2018.
View at: Google Scholar
C. Yeates, M. R. Gillings, A. D. Davison, N. Altavilla, and D. A. Veal, “PCR amplification of crude microbial DNA extracted from soil,” Letters in Applied Microbiology, vol. 25, no. 4, pp. 303–307, 1997.
View at: Publisher Site | Google Scholar
T. A. Hall, “BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT,” Nucleic Acids Symposium Series, vol. 41, pp. 95–98, 1999.
View at: Google Scholar
S. Kumar, G. Stecher, and K. Tamura, “MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets,” Molecular Biology and Evolution, vol. 33, no. 7, pp. 1870–1874, 2016.
View at: Publisher Site | Google Scholar
K. Tamura and M. Nei, “Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees,” Molecular Biology and Evolution, vol. 10, no. 3, pp. 512–526, 1993.
View at: Publisher Site | Google Scholar
J. M. Andrews, “Determination of minimum inhibitory concentrations,” Journal of Antimicrobial Chemotherapy, vol. 48, no. 1, pp. 5–16, 2001.
View at: Publisher Site | Google Scholar
S. Kim, J. Chen, T. Cheng et al., “PubChem 2019 update: improved access to chemical data,” Nucleic Acids Research, vol. 8, pp. 1102–1109, 2019.
View at: Google Scholar
D. A. Filimonov, A. A. Lagunin, T. A. Gloriozova et al., “Prediction of the biological activity spectra of organic compounds using the pass online web resource,” Chemistry of Heterocyclic Compounds, vol. 50, no. 3, pp. 444–457, 2014.
View at: Publisher Site | Google Scholar
G. V. de Melo Pereira, K. T. Magalhães, E. R. Lorenzetii, T. P. Souza, and R. F. Schwan, “A multiphasic approach for the identification of endophytic bacterial in strawberry fruit and their potential for plant growth promotion,” Microbial Ecology, vol. 63, no. 2, pp. 405–417, 2012.
View at: Publisher Site | Google Scholar
H. Panchal and S. S. Ingle, “Isolation and characterization of endophytes from the root of medicinal plant Chlorophytum borivilianum (Safed musli),” Journal of Advanced Research and Development, vol. 2, pp. 205–209, 2011.
View at: Google Scholar
S. Taghavi, C. Garafola, S. Monchy et al., “Genome survey and characterization of endophytic bacteria exhibiting a beneficial effect on growth and development of poplar trees,” Applied and Environmental Microbiology, vol. 75, no. 3, pp. 748–757, 2009.
View at: Publisher Site | Google Scholar
V. J. Szilagyi-Zecchin, A. C. Ikeda, M. Hungria et al., “Identification and characterization of endophytic bacteria from corn (zea mays L.) roots with biotechnological potential in agriculture,” AMB Express, vol. 4, Article ID 26, 2014.
View at: Publisher Site | Google Scholar
X. Q. Xiong, H. D. Liao, J. S. Liu et al., “Isolation of a rice endophytic bacterium, Pantoea sp. Sd-1, with ligninolytic activity and characterization of its rice straw degradation ability,” Letters in Applied Microbiology, vol. 58, no. 2, pp. 123–129, 2013.
View at: Publisher Site | Google Scholar
P. R. Hardoim, L. S. van Overbeek, G. Berg et al., “The hidden world within plants: ecological and evolutionary considerations for defining functioning of microbial endophytes,” Microbiology and Molecular Biology Reviews, vol. 79, no. 3, pp. 293–320, 2015.
View at: Publisher Site | Google Scholar
K. Kitahara and K. Miyazaki, “Revisiting bacterial phylogeny: natural and experimental evidence for horizontal gene transfer of 16S rRNA,” Mobile Genetic Elements, vol. 3, Article ID e24210, 2013.
View at: Google Scholar
A. J. Sabat, E. van Zanten, V. Akkerboom et al., “Targeted next-generation sequencing of the 16S-23S rRNA region for culture-independent bacterial identification-increased discrimination of closely related species,” Scientific Reports, vol. 7, Article ID 3434, 2017.
View at: Publisher Site | Google Scholar
P. M. Maela and M. H. Serepa-Dlamini, “Draft genome sequence of Stenotrophomonas pavanii strain MHSD12, a bacterial endophyte associated with Dicoma anomala,” Microbiology Resource Announcements, vol. 9, Article ID e00550, 2020.
View at: Publisher Site | Google Scholar
P. L. Ramos, S. van Trappen, F. L. Rocha et al., “Screening for endophytic nitrogen-fixing bacteria in Brazilian sugar cane varieties used in organic farming and description of Stenotrophomonas pavanii sp. nov,” International Journal of Systematic and Evolutionary Microbiology, vol. 61, no. 4, pp. 926–931, 2011.
View at: Publisher Site | Google Scholar
S. F. van Vuuren, “Antimicrobial activity of South African medicinal plants,” Journal of Ethnopharmacology, vol. 119, no. 3, pp. 462–472, 2008.
View at: Publisher Site | Google Scholar
M. B. Ticho, T. Morris, M. Meyer et al., “Antibacterial activity of rationally designed antimicrobial peptides,” International Journal of Microbiology, vol. 2020, Article ID 2131535, 9 pages, 2020.
View at: Publisher Site | Google Scholar
P. Chikezie, C. Ibegbulem, and F. Mbagwu, “Medicinal & aromatic plants medicinal potentials and toxicity concerns of bioactive principles,” Medicinal & Aromatic Plants, vol. 4, no. 3, pp. 1–15, 2015.
View at: Google Scholar
W. Johnson, W. F. Bergfeld, D. V. Belsito et al., “Safety assessment of benzyl alcohol, benzoic acid and its salts, and benzyl benzoate,” International Journal of Toxicology, vol. 36, no. 3, pp. 5S–30S, 2017.
View at: Publisher Site | Google Scholar
H. Diastuti, M. Chasani, and S. Suwandri, “Antibacterial activity of benzyl benzoate and crotepoxide from Kaempferia rotunda L. Rhizome,” Indonesian Journal of Chemistry, vol. 20, no. 1, pp. 9–15, 2020.
View at: Google Scholar
M. Singh, A. Kumar, R. Singh, and K. D. Pandey, “Endophytic bacteria: a new source of bioactive compounds,” 3 Biotech, vol. 7, pp. 1–14, 2017.
View at: Publisher Site | Google Scholar
A. B. Hsouna, M. Trigui, R. B. Mansour, R. M. Jarraya, M. Damak, and S. Jaoua, “Chemical composition, cytotoxicity effect and antimicrobial activity of Ceratonia siliqua essential oil with preservative effects against Listeria inoculated in minced beef meat,” International Journal of Food Microbiology, vol. 148, no. 1, pp. 66–72, 2011.
View at: Publisher Site | Google Scholar
A. A. Bharose and H. P. Gajera, “Antifungal activity and metabolites study of Bacillus strain against aflatoxin producing Aspergillus,” Journal of Applied Microbiology and Biochemistry, vol. 2, no. 2, pp. 1–8, 2018.
View at: Google Scholar
S. Yogeswari, S. N. Ramalakshmi, J. M. Muthu et al., “Identification and comparative studies of different volatile fractions from Monochaetia kansensis by GC-MS,” Global Journal of Pharmacology, vol. 6, pp. 65–71, 2012.
View at: Google Scholar
M. R. K. Rao, N. V. Lakshmi, and L. Sundaram, “Preliminary phytochemical and GC MS analysis of different extracts of psophocarpus tetragonolobus leaves,” Journal of Pharmaceutical Sciences, vol. 5, 2018.
View at: Google Scholar
A. Akpuaka, M. M. Ekwenchi, D. A. Dashak et al., “Biological activities of characterized isolates of N-hexane extract of Azadirachta indica A.juss (neem) leaves,” New York Science Journal, vol. 6, pp. 119–124, 2013.
View at: Google Scholar
P. Jishma, N. Hussain, R. Chellappan, R. Rajendran, J. Mathew, and E. K. Radhakrishnan, “Strain-specific variation in plant growth promoting volatile organic compounds production by five different Pseudomonas spp. as confirmed by response of Vigna radiata seedlings,” Journal of Applied Microbiology, vol. 123, no. 1, pp. 204–216, 2017.
View at: Publisher Site | Google Scholar
J. Rani and S. Giri, “Screening of bio-active compounds and anticancer activity of Punica granatum L,” World Journal of Science and Research, vol. 1, pp. 6–15, 2016.
View at: Google Scholar
M.-C. Cheng, Y.-B. Ker, T.-H. Yu, L.-Y. Lin, R. Y. Peng, and C.-H. Peng, “Chemical synthesis of 9(Z)-Octadecenamide and its hypolipidemic effect: a bioactive agent found in the essential oil of mountain celery seeds,” Journal of Agricultural and Food Chemistry, vol. 58, no. 3, pp. 1502–1508, 2010.
View at: Publisher Site | Google Scholar
D.-Y. Bang, I.-K. Lee, and B.-M. Lee, “Toxicological characterization of phthalic acid,” Toxicological Research, vol. 27, no. 4, pp. 191–203, 2011.
View at: Publisher Site | Google Scholar
P. Kushwaha, S. S. Yadav, V. Singh et al., “Phytochemical screening and GC-MS studies of the methanolic extract of Tridax procumbens,” International Journal of Pharmaceutical Sciences and Research, vol. 10, pp. 2492–2496, 2019.
View at: Google Scholar
Y. Hong, S. Huang, J. Wu et al., “Identification of essential oils from the leaves of 11 species of Eriobotrya,” Pakistan Journal of Botany, vol. 42, pp. 4379–4386, 2010.
View at: Google Scholar
V. Sharma, P. Kumar, and D. Pathak, “Biological importance of the indole nucleus in recent years: a comprehensive review,” Journal of Heterocyclic Chemistry, vol. 47, pp. 491–502, 2010.
View at: Google Scholar
M. Mulyono, B. W. Lay, O. L. Laora et al., “Antidiarrheal activity of apus bamboo (Gigantochloa apus) leaf extract and its bioactive compounds,” American Journal of Microbiology, vol. 4, no. 1, pp. 1–8, 2013.
View at: Publisher Site | Google Scholar
I. F. Begum, R. Mohankumar, M. Jeevan et al., “GC–MS analysis of bio-active molecules derived from paracoccus pantotrophus FMR19 and the antimicrobial activity against bacterial pathogens and MDROs,” Indian Journal of Microbiology, vol. 56, pp. 426–432, 2016.
View at: Google Scholar
M. A. Alfhili and M. H. Lee, “Triclosan: an update on biochemical and molecular mechanisms,” Oxidative Medicine and Cellular Longevity, vol. 2019, Article ID 1607304, 28 pages, 2019.
View at: Publisher Site | Google Scholar
M. M. Patil and S. S. Rajput, “Succinimides: synthesis, reaction and biological activity,” International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, pp. 8–14, 2014.
View at: Google Scholar

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Copyright © 2021 Sephokoane Cindy Makuwa and Mahloro Hope Serepa-Dlamini. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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