Diclofenac May Induce PIA-Independent Biofilm Formation in Staphylococcus aureus Strains

Table 1

Degree of degradation of biofilm induced by rifampicin (1/2 MIC) and diclofenac after treatment with sodium metaperiodate in 3 strains of S. aureus.

Strain	Control^a (OD570nm)	Rif^b		Rif + Dic^d		Dic^f
		OD570 nm	SMP^c (%)	OD570 nm	SMP^e (%)	OD570 nm	SMP^g (%)
A112	0.210	1.376	77.9	1.789	70.8	0.368	0.0
A150	0.219	1.263	78.0	1.376	67.9	0.525	0.0
B103	0.212	1.659	75.1	2.186	64.4	0.515	0.0

Rif, rifampicin; Dic, diclofenac (50 μg/mL); SMP, sodium metaperiodate; OD_{570 nm}, optical density at 570 nm. ^aStrain grown on tryptone soy broth (TSB) without supplementation; ^bstrain grown in TSB supplemented with rifampicin; ^cdegradation by metaperiodate (%) of biofilm induced by rifampicin; ^dstrain grown in TSB supplemented with rifampicin + diclofenac; ^edegradation by metaperiodate (%) of biofilm induced by rifampicin + diclofenac; ^fstrain grown in TSB supplemented with diclofenac; ^gdegradation by metaperiodate (%) of biofilm induced by diclofenac.