Diclofenac May Induce PIA-Independent Biofilm Formation in Staphylococcus aureus Strains

Table 2

Degree of degradation of biofilm induced by NaCl and diclofenac after treatment with sodium metaperiodate in 3 strains of S. aureus.

Strain	Control^a (OD570nm)	NaCl^b		NaCl + Dic^d		Dic^f
		OD570 nm	SMP^c (%)	OD570 nm	SMP^e (%)	OD570 nm	SMP^g (%)
A139	0.182	0.393	0.0	0.580	0.0	0.536	0.0
A150	0.177	0.889	69.4	0.513	29.7	0.386	0.0
B103	0.188	0.915	58.6	0.783	36,7	0.559	0.0

Dic, diclofenac (50 μg/mL); SMP, sodium metaperiodate; OD, optical density. ^aStrain grown on tryptone soy broth (TSB) without supplementation; ^bstrain grown in TSB supplemented with NaCl (3%); ^cdegradation by metaperiodate (%) of biofilm induced by NaCl; ^dstrain grown in TSB supplemented with NaCl + diclofenac; ^edegradation by metaperiodate (%) of biofilm induced by NaCl + diclofenac; ^fstrain grown in TSB supplemented with diclofenac; ^gdegradation by metaperiodate (%) of biofilm induced by diclofenac.