Molecular Detection of Antibiotic-Resistant Genes in Pseudomonas aeruginosa from Nonclinical Environment: Public Health Implications in Mthatha, Eastern Cape Province, South Africa

Table 1

Primer sequences for detection of gyrB genes.

Primers	Primers sequences (5′-3′)	Tm in 0°C	References
gyr B forward primer	CCT GAC CAT CCG TCG CCA CAA	55.3	[19]
Gyr B reverse primer	CGC AGC AGG ATG CCG ACG CC	53.1
Gyr B probe 1	FAM-CCG TGG TGG TAG ACC TGT TCC CAG ACC-BHQ		This study
Gyr B probe 2	FAM-CCG TGG TGG TAG ACC TGT TCC CAG ACC-BBQ