Molecular Detection of Antibiotic-Resistant Genes in Pseudomonas aeruginosa from Nonclinical Environment: Public Health Implications in Mthatha, Eastern Cape Province, South Africa

Table 2

rPCR cycle protocol.

Protocol	Temperature (°C)	Acquisition mode	Time	Ramp rate	Cycle
Denaturation	95	None	5 minutes	4.4	1
Quantification: Annealing	95	None	30 seconds	4.4	45
Extension	60	Single	1 minute	4.4
Cooling	40	None	30 seconds	4.4	1