Molecular Detection of Antibiotic-Resistant Genes in Pseudomonas aeruginosa from Nonclinical Environment: Public Health Implications in Mthatha, Eastern Cape Province, South Africa

Table 3

Primer sequences for detection of bla_CTX-M, bla_SHV, bla_TEM, bla_IMP, and bla_VIM genes.

Primers	Primers sequences (5′-3′)	Tm in 0°C	References
CTX-M forward primer	ATGAGYACCAGTAARGTKATGGC	58.7	[24]
CTX-M reverse primer	ATCACKCGGRTCGCCIGGRAT	59.3
CTX-M probe	FAM-CCCGACAGCTGGGAGACGAAACGT-BBQ	70.2
SHV forward primer	TCCCATGATGAGCACCTTTAAA	56.8	[25]
SHV reverse primer	TCCTGCTGGCGATAGTGGAT	58.6
SHV probe	FAM-TGCCGGTGACGAACAGCTGGAG-BBQ	68.3
TEM forward primer	GCATCTTACGGATGGCATGA	56.6	[25]
TEM reverse primer	GTCCTCCGATCGTTGTCAGAA	57.7
TEM probe	FAM-CAGTGCTGCCATAACCATGAGTGA-BHQ1	62.2
IMP forward primer	GGGCGGAATAGAGTGGCTTA	57.6	[26]
IMP reverse primer	GGCTTGAACCTTACCGTCTTTTT	59.3
IMP probe	FAM-CGATCTATCCCCACGTATGCATCTGAATTAACA-BHQ1	67.4
VIM forward primer	TGCGCTTCGGTCCAGTAGA	59.0	[26]
VIM reverse primer	TGACGGGACGTATACAACCAGAT	58.5
VIM probe	FAM-CTTCTATCCTGGTGCTGCGCATTCG-BHQ1	67.6