Campylobacter jejuni from Slaughter Age Broiler Chickens: Genetic Characterization, Virulence, and Antimicrobial Resistance Genes

Table 2

Primer sequences of virulence genes and PCR conditions used in this study.

Target gene	Primer	Primer sequence (5′ ⟶ 3′)	Conditions	Cycles	Size (bp)	References
cdtA	CDTA-FCDTA-R	CCTTGTGATGCAAGCAATC ACACTCCATTTGCTTTCTG	94°C for 15 min, 94°C for 1 min, 49°C for 1 min, and 72°C for 1 min, 72°C for 7 min	45	370	[39]
cdtB	CDTB-FCDTB-R	GTTAAAATCCCCTGCTATCAACCA GTTGGCACTTGGAATTTGCAAGGC	94°C for 15 min, 94°C for 1 min, 51°C for 1 min, and 72°C for 1 min, 72°C for 7 min	45	495	[39]
cdtC	CDTCFCDTCR	CGATGAGTTAAAACAAAAAGATA TTGGCATTATAGAAAATACAGTT	94°C for 15 min, 94°C for 1 min, 48°C for 1 min, and 72°C for 1 min, 72°C for 7 min	45	182	[39]
cadF	cadF-F2BcadF-R1B	TTGAAGGTAATTTAGATATGCTAATACCTAAAGTTGAAAC	95°C for 3 min, 94°C for 30 s, for 30 s, 43°C and 72°C for 1 min, 72°C for 5 min	45	400	[10]
ciaB	CIAB-652CIAB R1159	TGCGAGATTTTTCGAGAATGTGCCCGCCTTAGAACTTACA	95°C for 3 min, 94°C for 30 s, for 30 s, 54°C and 72°C for 1 min, 72°C for 5 min	45	527	[38]
pldA	PLDA-FPLDA-R	AAGAGTGAGGCGAAATTCCAGCAAGATGGCAGGATTATCA	95°C for 3 min, 94°C for 30 s, for 30 s, 46°C and 72°C for 1 min, 72°C for 5 min	45	385	[38]