General Phenotype of NADase Induction by CLI Treatment in Streptococcus pyogenes

Table 1

NADase activity of culture supernatant after administration of CLI in S. pyogenes strains.

Strain	emm type	NADase activity (U) without CLI^a	CLI-dependent NADase induction^b	Subinhibitory dose (μg/ml)	STSS/non-STSS	Reference
(CLI-sensitive)
1529	1	13.1	+ (3.2)	2^–6	STSS	[14]
11T-3	1	7.33	+ (5.7)	2^–6	Non-STSS	[16]
10–85	1	4.51	+ (2.9)	2^–6	STSS	[14]
11–171	1	6.6	+ (3.1)	2^–6	STSS	[16]
12–5	1	8.16	+ (2.8)	2^–5	STSS	[14]
ncu6B	3	3.91	+ (2.4)	2^–6	Non-STSS	This study
ncu62A	3	34.1	+ (1.4)	2^–6	STSS	This study
SSI-1	3	82.8	- (N/A)	2^–6	STSS	[13]
13-O-8	3	58.6	- (N/A)	2^–6	Non-STSS	This study
13-O-10	3	39.4	- (N/A)	2^–6	Non-STSS	This study
15T-11	3	49.5	- (N/A)	2^–6	Non-STSS	This study
ncu33A	4	18.4	+ (2.2)	2^–6	Non-STSS	This study
ncu115B	4	25.8	+ (1.7)	2^–6	Non-STSS	This study
ncu117B	4	36.0	+ (1.5)	2^–6	Non-STSS	This study
NU-syoni	6	16.0	+ (1.7)	2^–7	STSS	This study
ncu44A	12	3.4	+ (1.9)	2^–6	Non-STSS	This study
ncu47A	12	4.1	+ (1.6)	2^–6	Non-STSS	This study
ncu69A	12	4.4	+ (2.6)	2^–6	Non-STSS	This study
ncu28A	28	7.2	+ (2.6)	2^–6	STSS	This study
Seibu	89	2.2	+ (3.2)	2^–6	STSS	This study
ncu52A	89	8.15	+ (1.7)	2^–7	Non-STSS	This study
ncu60A	89	7.42	+ (2.4)	2^–6	STSS	This study
ncu61A	89	2.53	+ (3.8)	2^–5	Non-STSS	This study
(CLI-resistant)
D2TY	1	12.9	+ (4.6)	2^8	STSS	[17]
11–174	12	1.30	+ (5.1)	2^8	STSS	This study
ncu21A	12	1.72	+ (9.4)	2^8	STSS	This study
ncu55A	12	1.14	+ (8.5)	2^8	Non-STSS	This study

^aBasal (in the absence of CLI) levels of NADase activities. Bacteria were cultured without CLI. U: Units, one unit of NADase activity is defined as the amount (μg) of β-NAD cleaved per hour per μl culture supernatant, as described previously [10]. ^bWhether the extracellular NADase activity of each strain was increased with CLI treatment (+) or not (−) was determined based on the experimental results shown in Figure 1 to 6. CLI-dependent NADase activity induction was expressed as values of maximum fold-induction by CLI in parentheses. N/A, not applicant.