Influence of Fetuin-A on Chlamydia muridarum Pulmonary Infection

Figure 1

AHSG mice are susceptible to chlamydial infection. (a) Weight change as a function of days post Cm challenge. Age-matched wild-type (WT) C57BL/6 and fetuin-A-deficient AHSG mice (n = 6 per group) were challenged intranasally with 500 IFU Cm and monitored for weight change for 12 days. Mock (sucrose-phosphate-glutamate buffer) challenged mice were used as control. (b) Bacterial burden. At indicated days, the number of viable Cm in the lungs (n = 4 per group) was determined by infecting McCoy cell cultures with lung tissue homogenate for visualization of inclusions by fluorescence microscopy (cf., Materials and Methods). Weight change and Chlamydia IFU data represent the mean ± SD. Differences in weight change between the same strain of Cm-infected and mock-treated mice were analysed by 2-way ANOVA with Tukey’s multiple comparison test. , , .

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