Cellulosic Ethanol Production Using a Dual Functional Novel Yeast

Table 1

A survey of cellulosic ethanol production using β-glucosidase-producing strains without the addition of extra β-glucosidase.

Strain	Substrate	Fermentation apparatus	Solids loading (%)	Cellulase dosage	Time (h)	Ethanol titer (g/L)	Reference
S. cerevisiae NAN-227	Corncobs	Flask	7	^b20 IU/g solids	72	20	Shen et al. [14]
S. cerevisiae D56	Avicel	Flask	^a8	^c25 FPU/g cellulose	96	26.37	Lee et al. [13]
S. cerevisiae INVSc1	Japanese cedar	Tube	10	^d15 mg protein/g solids	72	12	Treebupachatsakul et al. [22]
S. cerevisiae SyBE001603	Avicel	Flask	^a4	^e10 FPU/g glucan	144	15.8	Hu et al. [23]
Clavispora NRRL Y-50464	Corncob residue	Bottle	20	^c0.15 ml/g solids	72	22	Liu et al. [16]
Clavispora NRRL Y-50464	Corncob residue	Bottle	20	^c0.15 ml/g solids	120	26.6	Liu et al. [16]
Clavispora NRRL Y-50464	Corncob residue	Bioreactor	25	^c0.2 ml/g solids	120	23	Liu et al. [16]
Clavispora NRRL Y-50464	Avicel	Bottle	^a14	^c15.3 FPU/g cellulose	72	40.44	Liu and Cotta [21]
Clavispora NRRL Y-50464	Avicel	Bottle	^a14	^c15.3 FPU/g cellulose	120	47	Liu and Cotta [21]
Clavispora NRRL Y-50464	SigmaCell	Bottle	^a14	^c15.3 FPU/g cellulose	72	39.64	Liu and Cotta [21]
Clavispora NRRL Y-50464	SigmaCell	Bottle	^a14	^c15.3 FPU/g cellulose	120	48	Liu and Cotta [21]
Clavispora NRRL Y-50464	Standard NREL corn stover	Bottle	25	^c15.3 FPU/g cellulose	72	17.2	Liu and Cotta [21]
Clavispora NRRL Y-50464	Standard NREL corn stover	Bottle	25	^c15.3 FPU/g cellulose	120	23	Liu and Cotta [21]
Clavispora NRRL Y-50464	Delignified NREL corn stover	Bottle	25	^c15.3 FPU/g cellulose	72	28.2	Liu and Cotta [21]
Clavispora NRRL Y-50464	Standard NREL corn stover	Bioreactor/Conventional	20	^c15.3 FPU/g cellulose	48	32	Liu and Cotta [21]
Clavispora NRRL Y-50464	Standard NREL corn stover	Bioreactor/Conventional	20	^c15.3 FPU/g cellulose	120	34.7	Liu and Cotta [21]
Clavispora NRRL Y-50464	Rice straw	Bottle	15	^c9 FPU/g solids	36	25	Chapla et al. [24]
Clavispora NRRL Y-50464	Rice straw	Reactor/Bottle	20	12 FPU cellic CTec2	24	19.7	Kumar et al. [25]
Clavispora NRRL Y-50464	Rice straw	Reactor/Bottle	20	12 FPU cellic CTec2	36	36.7	Kumar et al. [26]
Clavispora NRRL Y-50464	Conventional corn stover	Bioreactor/Helical stirring	25	^f5 mg protein/g glucan (6.7 FPU/g glucan)	72	35	Geberedikan et al. [20]
Clavispora NRRL Y-50464	Conventional corn stover	Bioreactor/Helical stirring	25	^f5 mg protein/g glucan (6.7 FPU/g glucan)	96	38.1	Geberedikan et al. [20]

^aCommercially available pure cellulose. ^bCellulase JU-A10 containing 4.3 IU/mL of filter paper activity and 0.8 IU/mL of β-glucosidase. ^cCellulase Celluclast 1.5 L. ^dCellulase from Trichoderma reesei culture supernatant. ^eCellulase Celluclasta. ^fCellulase Youtell #7.