International Journal of Microbiology

Research Article

High-Resolution Melting Analysis to Detect Antimicrobial Resistance Determinants in South African Neisseria gonorrhoeae Clinical Isolates and Specimens

Table 1

Oligonucleotides used in this study.
TargetPrimer 5′-3′
porA_FF_CCGTGCGTTACGATTCCCCC
porA_RR_ACAGCCGGAACTGGTTTCATCTG
blaTEM_FF_ATAGACAGATCGCTGAGATAGGTGC
blaTEM_RR_AAAAGCGGTTAGAGCGGCTATTG
tetM_FF_CCAGCCCCGTCGTCCAAATAGTC
tetM_RR_GCATCAATCATTTGCTCATGTGGC
penA_FF_CCGTGTGATTGTGGCGGTAACC
penA_RR_TGCCCAAGATGTTCAGGCTGC
G545SF_GCCGACTGCAAACGGTTACTACA
mosaicF_GCCGACTGCAAACGGTTACTACG
ponA_FF_GAGCGGTCGATAATGAGAAAATGG
ponA_RR_GCATCCAGCGAAACCAAAGC
L421PF_GGTGGTTCAAGAGCCGTTGCC
porB_FF_CAACAAACAATCCTTCGTCGGCTTG
porB_RR_GGCAAATTCGGGAGAATCGTAGCG
G120DF_CAGCCCCCTGAAAAACACCGA
G120KF_CAGCCCCCTGAAAAACACCA
A121GF_GGATTCCCAAGCATTGACGTTGCC
A121DF_GGATTCCCAAGCATTGACGTTGT
rpsJ_FF_GCGTTTCAACATTTTGCGTTCTCC
rpsJ_RR_CATCGGTAGTTTTATCGGTCCAATCC
V57MF_AACATTTTGCGTTCTCCGCACA
gyrA_FF_AAAATAACTGGAATGCCGCCTAC
gyrA_RR_GAAGTTGCCCTGTCCGTCTATC
S91FF_TACCACCCCCACGGCGATTT
D95AF_CGCCATACGGACGATGGTGG
D95GF_CGCCATACGGACGATGGTGCC
parC_FF_CGTGGTCGGCGAGATTTTGG
parC_RR_CGAACCGAAGTTGCCGATGC
S87RF_TACCATCCGCACGGCGACC
S88PF_CATCCGCACGGCGACAGTC
16S rRNA_FF_AGCCGTAACACAGGTGCTGC
16S rRNA_RR_GACCATTGTATGACGTGTGAAGCC
C1192UF_ATAAGGGCCATGAGGACTTGACA
mtrR_FF_GGGTTTCATTATACATACACGATTGC
mtrR_RR_GATGTCGTCGCAGATACGTTGG
G45DF_TTTGAAATGCCAATAGAGCGCGT
For SNPs, a common reverse primer was used from the chromosomal gene primer set.