International Journal of Microbiology

Research Article

High-Resolution Melting Analysis to Detect Antimicrobial Resistance Determinants in South African Neisseria gonorrhoeae Clinical Isolates and Specimens

Table 4

Evaluation of high-resolution melt assay for the detection of antimicrobial resistance determinants in Neisseria gonorrhoeae clinical isolates compared to direct swab specimens.
TargetIsolates (n = 22)Direct specimens (n = 22)Concordance (%)
Present n (%)Absent n (%)Present n (%)Absent n (%)
ponA_L421P13/22 (59.1)9/22 (40.9)12/22 (54.5)10/22 (45.5)95.5
por_G120D4/22 (18.2)18/22 (81.8)3/22 (13.6)19/22 (86.4)95.5
porB_G120K0/2222/22 (100)0/2222/22 (100)100
porB_A121G2/22 (9.1)20/22 (90.1)2/22 (9.1)20/22 (90.1)100
porB_A121D8/22 (36.4)14/22 (63.6)6/22 (27.3)16/22 (72.7)90.9
mtrR_G45D0/2222/22 (100)0/2222/22 (100)100
rpsJ_V57M22/22 (100)0/2221/22 (95.5)1/22 (4.5)95.5
gyrA_S91F18/22 (81.8)4/22 (18.2)18/22 (81.8)4/22 (18.2)100
gyrA_D95A6/22 (27.3)16/22 (72.7)5/22 (22.7)17/22 (77.3)95.5
gyrA_D95G3/22 (13.6)19/22 (86.4)3/22 (13.6)19/22 (86.4)100
parC_S87R0/2222/22 (100)0/2222/22 (100)100
parC_S88P0/2222/22 (100)0/2222/22 (100)100
16S_C1192U0/2222/22 (100)0/2222/22 (100)100
penA_G545S0/2222/22 (100)0/2222/22 (100)100
penA_mosaic0/2222/22 (100)0/2222/22 (100)100