Optimization and Chemical Characterization of Biosurfactant Produced from a Novel Pseudomonas guguanensis Strain Iraqi ZG.K.M

Table 1

Factors considered for optimization of biosurfactant production from identified strain.

Factor	Range
Incubation temperature	10, 15, 20, 25, 30, 35, 40, 45, and 50°C
pH	4, 5, 6, 7, 8, 9, 10, and 11
Carbon source	Crude oil, diesel, engine oil, glucose, olive oil, sunflower oil, sesame oil, and coconut oil
Carbon source concentration	0.5, 1, 2, 3, 4, and 5% (w/v)
Nitrogen sources	(NH4)2 SO4, NaNO3, NH4NO3, NH4Cl, yeast extract, and urea
Nitrogen sources concentration	0.5, 1, 2, 3, and 4% (w/v)
Salt concentration	0.5, 1, 2, 3, and 4% (w/v)
Incubation period	24, 48, 72, 96, 168, 192, 216, 240, and 264 h