Optimization and Chemical Characterization of Biosurfactant Produced from a Novel Pseudomonas guguanensis Strain Iraqi ZG.K.M
Table 2
Secondary screening of bacterial isolates for biosurfactant production.
No.
Isolate symbol
Drop collapse assay
Oil spreading assay (mm)
Surface tension
Emulsification index E24 (%)
BATH assay (%)
1
Z 4
+
—
62.67 ± 0.33
13 ± 1
20.33 ± 0.33
2
Z 5
+
48.33 ± 1.76
52
49 ± 1
66.33 ± 0.88
3
Z 7
+
33.33 ± 1.66
48.67 ± 0.67
43.66 ± 0.33
58.33 ± 0.88
4
Z 9
+
—
69.33 ± 0.33
—
—
5
Z 11
+
10.66 ± 0.66
67
—
12.66 ± 0.88
6
Z 14
+
7.66 ± 0.88
60.33 ± 0.33
—
15.33 ± 1.20
7
Z 25
+
30.33 ± 1.76
59
50.33 ± 0.33
74 ± 2.08
8
Z 31
+
77.66 ± 2.96
49
61
76.33 ± 0.88
9
Z 33
+
44.33 ± 0.33
52 ± 1
49.66 ± 1.45
64.33 ± 1.85
10
Z 34
+
—
68.33 ± 0.33
—
—
11
Z 35
+
23.33 ± 2.18
59
16.66 ± 0.33
30.33 ± 1.33
12
Z 36
+
10 ± 1.52
62.67 ± 0.33
10.33 ± 0.88
18.66 ± 1.76
13
Z 40
+
21.66 ± 0.33
61.67 ± 0.33
15
29.66 ± 0.88
14
Z 43
+
26.33 ± 0.33
60.67 ± 0.33
22.66 ± 0.33
56.66 ± 0.33
15
Z 44
+
—
61.33 ± 0.33
—
9.66 ± 0.66
16
Z 47
+
77.66±0.33
44
64.66±0.33
88.33±2.18
17
Z 48
+
73.33 ± 1.20
49
50.66 ± 0.66
71.33 ± 0.88
18
Z 49
+
39.33 ± 0.33
61
47.33 ± 0.33
56.33 ± 1.20
19
Z 50
+
9.33 ± 1.66
59 ± 1.73
32.66 ± 1.20
57 ± 1
20
Z 51
+
3.66 ± 1.33
69
—
9.66 ± 1.20
21
Z 52
+
12.66 ± 1.20
67
9.66 ± 0.66
10.66 ± 0.33
22
Z 53
+
27.33 ± 1.85
64.67 ± 0.33
22.66 ± 0.88
11.66 ± 1.33
23
Z 54
+
48.33 ± 0.33
56.33 ± 0.33
44.33 ± 0.33
65.66 ± 1.85
24
Z 56
+
39 ± 1.52
61
48.66 ± 0.33
58.66 ± 0.33
25
Z 60
+
36.33 ± 0.33
59.33 ± 0.67
26.66 ± 0.33
51.33 ± 0.33
26
Z 66
+
—
65
—
14.33 ± 0.66
27
Z 71
+
38 ± 2.08
55.33 ± 0.67
37.66 ± 0.33
5.66 ± 0.66
28
Z 77
+
49.33 ± 0.33
50.33 ± 0.33
36.33 ± 0.66
61.66 ± 2.02
29
Z 80
+
22 ± 2.08
59
10.66 ± 0.33
30.66 ± 1.20
Data: mean ± SEM. The experiments were performed in three replicates. The bold values indicate that Z47 showed the higher E24% and the lowest surface tension, so, it is the best biosurfactant producer.
