Research Article
Emergence of New Delhi Metallo-β-Lactamase (NDM) Genes Detected from Clinical Strains of Escherichia coli Isolated in Ouagadougou, Burkina Faso
Table 1
Primers used for detection of carbapenemases genes in E. coli.
| Target | Sequence (5′ ⟶ 3′) | Annealing temp. °C | Amplicon size (bp) | References |
| IMI | F: CTACGCTTTAGACACTGGC | 57 | 481 | [16] | R: AGGTTTCCTTTTCACGCTCA |
| KPC | F: CTGTCTTGTCTCTCATGGCC | 60 | 796 | [18–20] | R: CCTCGCTGTRCTTGTCATCC | OXA-48 | F: TTGGTGGCATCGATTATCGG R: GAGCACTTCTTTTGTGATGGC | 58 | 744 | [18] |
| IMP | F: GTTTATGTTCATACWTCG | 45 | 432 | [21, 22] | R: GGTTTAAYAAAACAACCAC |
| NDM | F: TGGCAGCACACTTCCTATC | 58 | 488 | [23] | R: AGATTGCCGAGCGACTTG |
| VIM | F: AGTGGTGAGTATCCGACAG | 52 | 212 | [23] | R: TCAATCTCCGCGAGAAG |
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