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PCR name | Targeted gene | Primer name | Sequence (5′ to 3′ end) | Amplicon size (bp) | Melting temperature (°C) | Reference |
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Multiplex 1 | blaKPC type | Forward | TGTTGCTGAAGGAGTTGGGC | 340 | 56 | [15] |
Reverse | ACGACGGCATAGTCATTTGC |
blaOXA-48-like including OXA-199 and OXA-370 | Forward | AACGGGCGAACCAAGCATTTT | 597 | [15] |
Reverse | TGAGCACTTCTTTTGTGATGGCT |
RepA | Forward | GACATTGAGTCAGTAGAAGG | 905 | [16] |
Reverse | CGTGCAGTTCGTCTTTCGGC |
KPC | Forward | CTGTCTTGTCTCTCATGGCC | 796 | [17] |
Reverse | CCTCGCTGTGCTTGTCATCC |
|
Multiplex 2 | blaVIM type | Forward | CGCGGAGATTGARAAGCAAA | 247 | 56 | [15] |
Reverse | CGCAGCACCRGGATAGAARA |
VIM2 | Forward | ATGTTCAAACTTTTGAGTAAG | 801 | [18] |
Reverse | CTACTCAACGACTGAGCG |
NDM-1 | Forward | CATTTGCGGGGTTTTTAATG | 998 | [19] |
Reverse | CTGGGTCGAGGTCAGGATAG |
|
Multiplex 3 | blaNDM-1 | Forward | TAAAATACCTTGAGCGGGC | 439 | 52 | [15] |
Reverse | AAATGGAAACTGGCGACC |
blaIMP variants except, IMP-3, IMP-16, IMP-27, IMP-31, IMP-34, and IMP-35 | Forward | GAGTGGCTTAATTCTCRATC | 183 | [20] |
Reverse | CCAAACYACTASGTTATCT |
blaOXA-23-like | Forward | GTGGTTGCTTCTCTTTTTCT | 736 | [15] |
Reverse | ATTTCTGACCGCATTTCCAT |
|
Multiplex 4 | OXA-58-like | Forward | TGGCACGCATTTAGACCG | 507 | 52 | [21] |
Reverse | AAACCCACATACCAACCC |
VIM1 | Forward | AGTGGTGAGTATCCGACAG | 261 | [20] |
Reverse | ATGAAAGTGCGTGGAGAC |
NDM | Forward | GGTTTGGCGATCTGGTTTTC | 621 | [21] |
Reverse | CGGAATGGCTCATCACGATC |
|
Multiplex 5 | OXA-23-like | Forward | GATCGGATTGGAGAACCAGA | 501 | 52 | [21] |
Reverse | ATTTCTGACCGCATTTCCAT |
OXA-24-like | Forward | TTCCCCTAACATGAATTTGT | 1024 | [21] |
Reverse | GTACTAATCAAAGTTGTGAA |
OXA-51-like | Forward | TAATGCTTTGATCGGCCTTG | 353 | [21] |
Reverse | TGGATTGCACTTCATCTTGG |
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