The Relationship between the Biofilm Genes and Antibiotic Resistance in Stenotrophomonas maltophilia

Table 1

Primers used in this study.

Primers	Sequences (5′–3′)	Cycles	Initial denaturation	Cycling	Final extension	Length (bp)	References
23S rRNA	F: CAGCCTGCGAAAAGTA; R: TTAAGCTTGCCACGAAC	30	5 min, 95°C	30 s, 94°C; 45 s, 54°C; 1 min, 72°C	7 min, 72°C	520	[16]
smf-1	F: GGAAGGTATGTCCGAGTCCG; R: GCGGGTACGGCTACGATCAGTT	30	5 min, 95°C	45 s, 94°C; 45 s, 62°C; 45 s, 72°C	7 min, 72°C	674	[16]
rmlA	F: GCAAGGTCATCGACCTGG; R: TTGCCGTCGTAGAAGTACAGG	30	5 min, 95°C	20 s, 94°C; 20 s, 60°C; 30 s, 72°C	10 min, 72°C	82	[16]
spgM	F: GCTTCATCGAGGGCTACTACC; R: ATGCACGATCTTGCCGC	30	5 min, 95°C	20 s, 94°C; 20 s, 60°C; 30 s, 72°C	10 min, 72°C	80	[16]
rpfF	F: CTGGTCGACATCGTGGTG; R: TGATCCGCATCATTTCATGC	36	5 min, 94°C	45 s, 94°C; 45 s, 59°C; 45 s, 72°C	5 min, 72°C	151	[16]
L1	F: AGCCGTTAAAATTAAGCCC; R: CTTGATTGAAGGGTTGGGCG	36	5 min, 94°C	45 s, 94°C; 45 s, 56°C; 45 s, 72°C	5 min, 72°C	909	[11]
L2	F: CGATTCCTGC AGTTCAGT; R: CGGTTACCTC ATCCGATC	36	5 min, 94°C	30 s, 94°C; 30 s, 55°C; 30 s, 72°C	5 min, 72°C	870	[17]
sul1	F: ATGGTGACGGTGTTCGGCATTCTGA; R: CTAGGCATGATCTAACCCTCGGTC	36	5 min, 94°C	45 s, 94°C; 45 s, 62°C; 45 s, 72°C	5 min, 72°C	840	[11]
sul2	F: GAAGCGCAGCCGCAATTCAT; R: CCTGTTTCGTCCGACACAGA	36	5 min, 94°C	45 s, 94°C; 45 s, 60°C; 45 s, 72°C	5 min, 72°C	435	[11]
sul3	F: GAGCAAGATTTTTGGAATCG; R: CATCTGCAGCTAACCTAGGGCTTTGGA	30	5 min, 94°C	60 s, 94°C; 60 s, 55°C; 1 min, 72°C	7 min, 72°C	799	[18]
Smqnr	F: ACACAGAACGGCTGGACTGC; R: TTCAACGACGTGGAGCTGT	36	5 min, 94°C	45 s, 94°C; 45 s, 59°C; 45 s, 72°C	5 min, 72°C	818	[19]
dfrA13	F: CTCATCTGCTGGCTATCTCA; R: GAAACTATCACTAATGGCAGC	36	5 min, 94°C	45 s, 94°C; 45 s, 59°C; 45 s, 72°C	5 min, 72°C	641	[20]