Recurrent Focal Segmental Glomerulosclerosis: A Discrete Clinical Entity

Figure 2

Plasmapheresis effluent from a patient with a recurrent FSGS disrupts the cytoskeleton of human podocytes in culture. Immortalized human podocytes (gift from Dr. Saleem) were incubated for 6 hours with 10% plasmapheresis effluent from a control patient undergoing plasmapheresis for a nonrenal disease (upper panel); PPE from a patient with recurrent FSGS collected at the start of procedure (middle panels, “early PPE”); end of plasmapheresis (lower panels, “late PPE”). The “early PPE,” but not the late PPE FSGS sample disrupts polymerized actin (phalliodin staining, red) and nonmuscle mysosin II (staining with anti-MYH9 antibody-green); arrowheads: actin stress fibers; scale bar 5 mc.